Components

Question 1

Expiration date of and RBC when only anticoagulant is added. Exception to this?

Answer 1

21 days
Unless CPDA 1 is used then 35 days

Question 2

MLs of anticoagulant per 100mL of blood

Answer 2

14mL per 100mL of WB
70 in one unit with 500mL of WB

Question 3

Different Additive solutions

Answer 3

AS 1, 3, 5 and 7
1 and 3 are the most common

Question 4

Additive solution with no mannitol

Answer 4

AS3

Question 5

Required viability for a unit with AS added

Answer 5

75% viability 24 hrs post tx

Question 6

Expiration date of a unit after AS

Answer 6

42 days

Question 7

What kind of cells do you see when RBC membrane is lost

Answer 7

Spherocytes

Question 8

How do RBCs generate energy

Answer 8

Anaerobically, from glucose

Question 9

What decreases during storage for RBC storage lesion

Answer 9

Ph
Glucose consumption
ATP
Plasma Na+
2,3 DPG
Deformability

Question 10

What increases during storage for RBC storage lesion

Answer 10

Lactic acid
Plasma K+
RBC Na*
Osmotic fragility
Adherence/interaction with endothelial cells

Question 11

RBCs storage temp

Answer 11

1-6 C

Question 12

Which ABO can be transfused for whole blood

Answer 12

Must be type specific

Question 13

Exp date of RBC unit if system is open

Answer 13

24 hrs

Question 14

QC for leukoreduction

Answer 14

Less than 5.0x10^6
But at least 85% of RBCs retained

Question 15

Standard bump in HdB from one unit

Answer 15

1 g/dL increase in HgB or 3% Hct per unit

Question 16

Deferral period for whole blood donation

Answer 16

8 weeks

Question 17

Deferral period for aphaeresis double unit donation

Answer 17

16 weeks

Question 18

QC for washing units

Answer 18

80% of RBCs to remain after washing

Question 19

How long after expiration can a unit be rejuvenated

Answer 19

Up to 3 days after expiration

Question 20

How long must a unit be transfused or frozen after rejuvenation

Answer 20

24 hrs

Question 21

Frozen RBC temp and exp

Answer 21

10 years at -65C or colder (high glycerol, most common)
10 years at -120C or colder (low glyc)

Question 22

How long is a thawed unit good for

Answer 22

If open system 24 hrs
If closed system 14 days

Question 23

QC for thawing RBCs

Answer 23

Must remain 80% of RBCs

Question 24

How long after collection must while blood derived platelets be harvested

Answer 24

Within 8 hours of collection

Question 25

How to collect platelets from WB spins

Answer 25

Soft spin then hard spin

Question 26

QC for pooled or WB derived platelets

Answer 26

Each dose must be 5.5x10^10 90% of the time. 6 doses are pooled to make one

Question 27

Exp for platelets

Answer 27

5 days

Question 28

Storage for platelets

Answer 28

20-24C with agitation

Question 29

pH requirement for platelets

Answer 29

Greater than or equal to 6.2

Question 30

Platelet exp date is system is opened

Answer 30

4 hours Also 4 hours if pooled using open system

Question 31

QC for single donor platelets apheresis

Answer 31

3.0x10^11 90% of the time

Question 32

QC for irradiation

Answer 32

25-50 gray to the center 15 gray to the rest

Question 33

What does irradiation do

Answer 33

T lymphocytes are incapable of replicating Prevents graft v host disease

Question 34

Exp date of irradiates unit

Answer 34

28 days or original if shorter

Question 35

Time for freezing fresh frozen plasma

Answer 35

Within 8 hours

Question 36

Time for freezing FP24

Answer 36

Within 24 hours

Question 37

Which type of plasma can cryo be manufactured from

Answer 37

FFP only

Question 38

FFP storage and exp

Answer 38

-18C or colder for 1 year Or -65C for 7 years

Question 39

Which clotting factors does FFP contain

Answer 39

All factors 1mL of FFP contains 1 unit of each clotting factor

Question 40

Storage temp for plasma after thawing

Answer 40

1-6C

Question 41

How long is thawed FFP good for

Answer 41

24 hours After that it can still be used for 5 days but it needs to be called thawed plasma (not FFP)

Question 42

Which factors are lower in plasma than FFP

Answer 42

Factor V and VIII

Question 43

When can liquid plasma be derived from WB

Answer 43

At any point during shelf life and up to 5 days after exp of unit

Question 44

Liquid plasma exp date

Answer 44

21 days

Question 45

From what and when is cryo manufactured

Answer 45

From FFP after it’s thawed at 1-6C

Question 46

How long after FFP is thawed must cryo be re frozen

Answer 46

1 hour

Question 47

What does cryo contain

Answer 47

Average of 250mg of fibrinogen, factor VIII, fibronectin, factor XIII and Von Willebrands factor

Question 48

Minimum cryo values

Answer 48

80 IU of factor VIII 150 fibrinogen

Question 49

Cryo storage temp and exp

Answer 49

-18C or colder for 1 year

Question 50

Cryo exp after thawing

Answer 50

6 hours closed system 4 hours open system Starts when unit hits thawer

Question 51

Storage temp for cryo after thawing

Answer 51

20-24 C NO agitation

Question 52

How are granulocytes collected

Answer 52

Leukopheresis

Question 53

Granulocytes exp and storage

Answer 53

24 hrs store 20-24 no agitation Best if given in 8 hrs

Question 54

Shipping temps for blood cryo FFP and platelets

Answer 54

RBCs 1-10 with wet ice FFP and Cryo frozen with dry ice Platelets 20-24 24 hrs max for delivery