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Histopathology > Dehydration > Flashcards

Dehydration Flashcards

1
Q

Process of removing intercellular and extracellular water from the tissue following fixation and prior to impregnation.

A

Dehydration

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2
Q

Removal of water by evaporation from a solid, semi-solid, or liquid.

A

Drying

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3
Q

Slow substitution of the water in the tissue with an organic solvent

A

Dehydration

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4
Q

True or False.

The amount of dehydrating agent in each step should not be greater than 10 times the volume of the tissue.

A

False.

(The amount of dehydrating agent in each step should not be less than 10 times the volume of the tissue)

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5
Q

What is the preferred concentration of alcohol?

A

70-90%

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6
Q

What is the most common dehydrating agent?

A

Alcohol

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7
Q

What is the alcohol concentration for delicate tissues like embryonic tissues?

A

30%

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8
Q

What is the usual duration when using alcohol for dehydration?

A

1-2 hours

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9
Q

How many changes will you do to ensure the complete removal of water when dehydrating with alcohol?

A

2

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10
Q

What is placed 1/4 inch deep in the bottom of the container to ensure complete dehydration?

A

anhydrous copper sulfate

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11
Q

What indicates the full saturation of dehydrating fluids with water?

A

blue discoloration of copper sulfate crystals

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12
Q

What type of alcohol-dehydrating agent is considered the best dehydrating agent whereby it mixes with water and many organic solvents?

A

Ethyl alcohol (ethanol)

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13
Q

True or False.

Never leave tissues in 95% or 100% ethanol for more than 2 hours.

A

True

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14
Q

What alcohol-dehydrating agent is considered the best dehydrating agent for blood smears?

A

Methyl alcohol

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15
Q

What alcohol-dehydrating agent is used for plant and animal micro-technique?

A

Butyl alcohol

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16
Q

What alcohol-dehydrating agent is used undiluted with water?

A

High Grade Alcohol

17
Q

What dehydrating agent is used for tissue perfusions however causes brittleness?

A

Acetone

18
Q

For how long does acetone dehydrates a tissue?

A

1/2 - 2 hours

19
Q

What dehydrating agent removes most lipids?

A

Acetone

20
Q

What dehydrating agent is referred to as an excellent dehydrating and clearing agent?

A

Diethylene dioxide

21
Q

What is the refractive index of diethylene dioxide?

A

1.42

22
Q

What is the boiling point of diethylene dioxide?

A

101.5℃

23
Q

What refers to the method whereby the tissue is wrapped in a gauze bag and suspended in a bottle containing dioxane and a little anhydrous calcium oxide?

A

Weiseberger’s Method

24
Q

What is the duration period of the dehydration when using Weiseberger’s method?

A

3-24hrs

25
Q

What dehydrating agents dehydrate rapidly, are nontoxic, have no harmful effect, and tissues can be stored in it for months without producing hardening or distortion?

A

Ethylene glycol monoethyl ether (cellusolve)

26
Q

What is the boiling point for cellusolve?

A

156.4℃

27
Q

What is the combustion point for ethylene glycol ethers?

A

110-120℃

28
Q

What dehydrating agent is not used anymore?

A

triethyl phosphate

29
Q

What is the boiling point for triethyl phosphate?

A

215℃

30
Q

What dehydrating agent is toxic if inhaled and ingested?

A

tetrahydrofuran (THF)

31
Q

What dehydrating agent is the most toxic dehydrating agent?

A

carbon tetrachloride

31
Q

What is the additive for 95% ethanol that softens tissues?

A

4% phenol

32
Q

In transmission electron microscopy, what dehydrating solvent solubilized lipid?

A

ethanol

33
Q

In transmission electron microscopy, what refers to the transition fluid that is very reactive even at low temperatures?

A

propylene oxide

34
Q

What is a good substitute for propylene oxide?

A

acetonitrile

Histopathology (11 decks)

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