Developmental Neurogenetics Part I Flashcards Preview

Developmental Genetics > Developmental Neurogenetics Part I > Flashcards

Flashcards in Developmental Neurogenetics Part I Deck (12):
1

How does a Drosophila egg become multicellular from being a single cell with one nucleus? Why does it do this?

- A single nucleus is unable to control an entire egg.
- Divide the nuclei, and then divide the large egg into multiple cells with the nuclei.
- These nuclei lie in the periphery.
- Cell membranes form between the nuclei to create individual cells.

2

What are cellular blastoderms? How are they formed?

- A layer of cells surrounding the yolk sac.
- Nuclei accumulate to the surface of the egg cell and are then encompassed by the cell membrane.
- These surround the yolk sac, single cellular fashion.

3

Define Drosophila Gastrulation

The formation of multiple cell layers.
- 3 germ layers.
- Endoderm, Ectoderm, Mesoderm.

4

Define Endoderm

- Innermost layer of cells or tissue of an embryo in early development, or the parts derived from this, which include the lining of the gut and associated structures.

5

Define Ectoderm

- Outermost layer of cells or tissue of an embryo in early development, or the parts derived from this, which include the epidermis, nerve tissue, and nephridia.

6

Define Mesoderm

- Middle layer of cells or tissues of an embryo, or the parts derived from this (e.g. cartilage, muscles, and bone).

7

In Drosophila embryos, how are neuroblasts selected for?

- Selected from patches of cells in the ectoderm expressing proneural genes.
- Delaminate to form the nervous system.
- Signals involving the Notch receptor protein and other 'neurogenic gene' products select for neuroblasts via lateral inhibition.

8

Define delaminate

- Separation into layers.
- /initial splitting of cells in the embryo.

9

Define neuroblasts

- Embryonic cell in which nerve fibres originate.

10

What is achaete? How was it found?

- Proneural gene.
- In situ transcript localisation. Showed via blue dye.
- Involved: DIG / Anti-DIG/ Alkaline Phosphatase / BCIP+ NBT / Antisense RNA.

11

Where do proneural genes occur?
(achaete)

Occur in patches, encompassing a number of cells.

12

How many cells from a patch of cells expressing the proneural gene achaete become neurons?

- One cell from the patch becomes a neuron and migrate.