Flashcards in Diagnosis of Bacterial and Fungal Infections Deck (18):
Which two choices of diagnostic method can you use to identify a pathogen?
Identification of the agent or identification of the host immune response
What are some limitations of direct microscopic examination?
Sensitivity of direct staining is poor; some bacteria cannot be stained
Nutrient broth, peptone broth, and thioglycolate broth are examples of _______ culture.
Liquid (broth media)
Trypticase soy w/blood, PEA, MacConkey, Sabouraud's destrose are examples of _____ culture.
Solid (agar media)
PEA agar is Gram-_______ selective media
MacConkey agar is Gram-_____ selective media
What is Sabouraud's dextrose agar used to culture
Uses mass spectrometry to measure a unique molecular fingerprint of an organism
Non-culture based from clinical samples used for identification of isolates
Enzyme immunoassay, agglutination, fluorescent antibody staining
HPLC, GLC, MALDI-TOF, analysis of total cell fatty acids/proteins
Limulus amoebocyte assay fro LPS
Which direct test for microbial components is adapted from clotting mechanism of a horseshoe crab?
Limulus amoebocyte assay
Detects humoral (Ab) response
Agglutination, precipitation, ELISA
Detects cell mediated immune response
Tuberculin reaction, interferon gamma test
Antibody development after exposure to a pathogen or an antigen
Measurement of antibody level (quantity)