Diagnosis of Bacterial and Fungal Infections Flashcards Preview

Bacteriology Exam 1 > Diagnosis of Bacterial and Fungal Infections > Flashcards

Flashcards in Diagnosis of Bacterial and Fungal Infections Deck (18):
1

Which two choices of diagnostic method can you use to identify a pathogen?

Identification of the agent or identification of the host immune response

2

What are some limitations of direct microscopic examination?

Sensitivity of direct staining is poor; some bacteria cannot be stained

3

Nutrient broth, peptone broth, and thioglycolate broth are examples of _______ culture.

Liquid (broth media)

4

Trypticase soy w/blood, PEA, MacConkey, Sabouraud's destrose are examples of _____ culture.

Solid (agar media)

5

PEA agar is Gram-_______ selective media

Positive

6

MacConkey agar is Gram-_____ selective media

Negative

7

What is Sabouraud's dextrose agar used to culture

Fungal culture

8

Uses mass spectrometry to measure a unique molecular fingerprint of an organism

MALDI-TOF

9

Non-culture based from clinical samples used for identification of isolates

PCR

10

Enzyme immunoassay, agglutination, fluorescent antibody staining

Antigen detection

11

HPLC, GLC, MALDI-TOF, analysis of total cell fatty acids/proteins

Chemical detection

12

Limulus amoebocyte assay fro LPS

Biological detection

13

Which direct test for microbial components is adapted from clotting mechanism of a horseshoe crab?

Limulus amoebocyte assay

14

Detects humoral (Ab) response

Agglutination, precipitation, ELISA

15

Detects cell mediated immune response

Tuberculin reaction, interferon gamma test

16

Antibody development after exposure to a pathogen or an antigen

Seroconversion

17

Measurement of antibody level (quantity)

Antibody titer

18

Compares acute and convalescent phase samples

Paired serum titers