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Flashcards in DNA Replication/Repair 1 Deck (15):
1

How many origins of replication for prokaryotes?

1. One

2

How many replication sites for eukaryotes?

1. Multiple

3

What are polymerases?

1. Enzymes that synthesize (add) nuclei acids to DNA strand

4

What are nucleases?

1. Enzymes that remove nucleotides from DNA strand

5

Difference between exonucleases and endonucleases?

1. Exonucleases: remove nucleotides from the 5’ or 3’ end of the strand

2. Endonucleases: remove nucleotides from the middle of a DNA strand

6

In what direction does DNA polymerase act and therefore synthesize nucleic acids?

5’ —-> 3’

7

In what direction is the DNA template read for synthesis ?

3’ ——> 5’

8

In what direction is proofreading done by exonucleases?

3’——-> 5’

9

Enzyme in charge of breaking hydrogen bonds between base pairs?

Helicase

10

Protein in charge of binding to DNA strands to prevent them from reassociating?

1. Single Stranded Binding Proteins (SSB)

11

What is primase for?

1. Synthesizing the RNA primer to initiate DNA synthesis and replication.

12

Differences in DNA polymerases between prokaryotes and eukaryotes? (In terms of Synthesis)

1. Prokaryotes: Polymerase III
2. Eukaryotes: Polymerases Alpha and Delta

13

Difference in removal of RNA primers between prokaryotes and eukaryotes?

1. Prokaryotes: Polymerase I
2. Eukaryotes: RNase H

14

It is formed by the Okazaki fragments and it’s called the _______ strand?

1. Lagging strand

15

How are Okazaki fragments sealed to one another ?

1. DNA ligase