Flashcards in effects of diff factors on enzyme activity Deck (9)
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1
Q
What reaction does catalase catalyse
A
2H2O2-> O2 +2H2O
2
Q
Describe the method of the practical(catalase)
A
- fill a bowl/tray w water and put gas burette in and set at zero.
- connect a manometer from base of gas burette to bung of conical flask.
- add sol with H2O2 and catalase into conical flask and immediately start a stopwatch.
- If temp isn’t being measured sol should be at 20 C
- At specific time intervals check volume of oxygen produced and put in table
- experiment can be stopped when level of O2 produced no loner increases
3
Q
what is dependant variable(catalase)
A
volume of O2 produced
4
Q
what variables should be controlled catalase
A
- type/origin of enzyme
- volume of mixture solution
- temp.
- PH
- Conc.
- conc. of enzyme(if varying substrate conc is being used)
- conc. of substrate(if varying enzyme conc is being used)
- equipment that’s used
- time intervals for O2
5
Q
How can the rate of reaction be worked out(catalase)
A
Volume of O2/Time
6
Q
What does alpha-amylase catalyse
A
Hydrolysis of starch into maltose
7
Q
How is the rate of reaction worked out(amylase)
A
decrease of starch per unit of time
8
Q
Describe the method of alpha-amylase Experiment
A
- prepare water bath at 30C
- put equal amounts of iodine/potassium iodide in a spotting tile.
3.form mixture of amylase and starch in
test tube and place in water bath
- At regular intervals using a pipette drop in spotting tile
- Note colour change,continue until no color change
9
Q
what is dependant variable in amylase experiment.
A
color change of iodine