Electrophoresis

Question 1

Electrophoresis

Answer 1

Movement of charged particles through the porous gel matric under the influence of electric field

Question 2

Name the two types of electrophoresis

Answer 2

Moving boundary electrophoresis
Zone electrophoresis

Question 3

What is moving boundary electrophoresis?

Answer 3

The electrophoresis that doesn’t need any supporting media and forms boundary

Question 4

What is zone electrophoresis

Answer 4

The electrophoresis that needs a supporting media and forms bands

Question 5

What are the two types of gel matrix?

Answer 5

Polyacrylamide
Agarose

Question 6

What is the correlation between concentration and the molecular weight of the sample?

Answer 6

Inversely proportional

Question 7

How is polyacrylamide formed?

Answer 7

Acrylamide + bisacrylamide

Question 8

Name the initiator and a catalyst in the formation of polyacrylamide chain.

Answer 8

Initiator - ammonium persulfate
Catalyst - N, N, N’, N’ - Tetramethylene diamine

Question 9

What is the concentration used in separation of protein in polyacrylamide gel?

Answer 9

10 to 15%

Question 10

Is angrose gel linear or branched polysaccharide?

Answer 10

Linear

Question 11

How is agarose gel formed?

Answer 11

Galactose and anhydrogalactose

Question 12

Name the algae used in the formation of agarose.

Answer 12

Gracilaria and Gelidium

Question 13

What is the concentration of agarose gel?

Answer 13

1 to 3%

Question 14

What are the stains used in agarose gel electrophoresis?

Answer 14

Ethidium bromide, DAPI, methylene blue

Question 15

Name the intercalating agent (or metal chelater) of agarose gel electrophoresis

Answer 15

Ethidium bromide

Question 16

What kind of detergent is sodium dodecyl sulphate?

Answer 16

Anionic detergent

Question 17

Name these tracking dye in SDS electrophoresis

Answer 17

Bromo phenol blue

Question 18

Write the movement order in SDS electrophoresis

Answer 18

Cl — Protein SDS — Glycinate

Question 19

Name the anionic dye that binds to the hydrophobic part of the protein

Answer 19

Coomasie brilliant blue

Question 20

Name the dye for the sensitive proteins of ng

Answer 20

Silver stain

Question 21

Name two reducing agents

Answer 21

B mercaptoethanol and dithiothretol

Question 22

In isoelectric focusing who create the pH gradient?

Answer 22

Polyampholyte

Question 23

Write the relation between the charge and isoelectric point of a protein

Answer 23

pI more than pH then positive
pI less than pH then negative
pI equals to pH then neutral

Question 24

In 2D electrophoresis how are the proteins separated?

Answer 24

First by pI and the by mass

Question 25

Why are DNA sequencing gel used?

Answer 25

To seperate single stranded DNA that are shorter than 1000nt and differing in size by 1nt

Question 26

Why is pulsed field electrophoresis used?

Answer 26

To separate molecules that are of size 2000kbp to 10Mbp

Question 27

What is the unique characteristic of pulsed field gel electrophoresis?

Answer 27

Uses electric field at an angle of 120°

Question 28

What can we use when the sample is sensitive to the ammonium persulfate?

Answer 28

Riboflavin

Question 29

Who is a neurotoxin in their monomer form?

Answer 29

Bisacrylamide and acrylamide

Question 30

Blue native PAGE

Answer 30

The technique that allows separation of proteins present in multiprotein complexes in a native confirmation with higher resolution

Question 31

Name the two buffer for the separation of nucleic acid

Answer 31

TBE and TAE

Question 32

Name the salt that creates favourable ionic strength and pH for the sample

Answer 32

Tris HCl

Question 33

Name the density ingredients of the gel

Answer 33

Glycerol or sucrose

Question 34

How is capillary electrophoresis of DNA is better than the normal electrophoresis?

Answer 34

Provides better speed, resolution, sensitivity and data handling