Enzymatic inhibition and Chemical Rxns week 5 test 2 Flashcards Preview

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Flashcards in Enzymatic inhibition and Chemical Rxns week 5 test 2 Deck (27):
1

competitive inhibition

inhibitor competes with substrate
Km moves to right
Vmax is unchanged

2

uncompetitive inhibition

inhibitor binds to enzyme substrate complex, but not enzyme alone
Km moves to the left
Vmax moves to down or is reduced

3

noncompetitive inhibition

inhibitor binds the enzyme or the enzyme-substrate complex
*slows or stops enzymatic process, substrate binding not affect
Km does not change
Vmax reduces

4

mix inhibition

inhibitor binds enzyme alone or enzyme substrate
**affects substrate binding and enzyme function
Km moves right
Vmax is reduced

5

tips to remembering the inhibitors

all of them reduce Vmax other than competitive
then remember the Km values and it is easy
Un is to the left
non is unchanged
mixed is to the right

6

what does free energy to?

performs work

7

chemical rxn

changes in molecular structure performed through work

8

if you have a negative delta G then what direction does the rxn go?

forward

9

is you have a positive delta G then what direction does the rxn go?

backwards or reverse same thing

10

what are the five types of biochemical rxns

1. making and breaking carbon bonds
2. Molecular RAR
3. Free radical Rxns
4. Group transfers (most abundant)
5. REDOX

11

two types of making and breaking carbon bonds

Condensation-two molecules join to create a larger molecule while forming a small molecule byproduct
Carboxylation/decarboxylation-the addition or removal or a carboxyl group

12

What is a free radical

a molecule containing an unpaired electron
superoxide dismutase removes free radicals from cells
Dopamine Quinone formation leads to production of free radicals
Vitamin E is an antioxidant

13

Five common group transfers and their

Phosphorylation
Ubiquitination
Acetylation
Methylation
Hydroxylation

14

enzyme for phosphorylation

kinase
ATP

15

enzyme for ubiquitination

ubiquintin lingase

16

enzyme for actelyation

acetyltransferase

17

enzyme for methylation

methyltransferase

18

enzyme for hydroxylation

hyroxylase

19

Why is magnesium important for ATP

stabilizes that ATP in an aqueous solution

20

What is the most common source of energy for group transfer rxns

ATP

21

At high ATP:ADP concentration what happens

you get more energy from breaking the phosphate bond than if you had a lower concentration of ATP to ADP

22

How much energy is the lowest amount of energy you can get from breaking 1 phosphate bond on ATP

-30.5 kj/mol

23

Does ATP provide energy by group transfer or hydrolysis mostly?

Via group transfer because this allows for use of energy further down the road in the rxns.

24

Phosphate transfer can also drive signal transduction true or false?

True vic cAMP

25

REDOX Rxns (movement of electrons)
reduction vs. oxidation

reduction requires energy and oxidation releases energy

26

what are four ways to transfer electrons

direct electron transfer
hydrogen atom transfer
hydride ions
combination with oxygen

27

why are REDOX rxns so important to biological systems

by breaking down larger molecules, electrons are then transferred to be used as an energy source via gradients in the ETC for produce ATP.