ENZYMES LABORATORY Flashcards
(215 cards)
1
Q
WHAT IS THE ENZYME NOMENCLATURE FOR ALP
A
ALKALINE PHOSPHATASE 3.1.3.1
2
Q
Major tissues of the ALP
A
Intestinal, Placenta, Bone, Liver
3
Q
Another name of ALP
A
Alkaline Orthophosphoric Monoester Phosphohydrolase
4
Q
It is involved in the hydrolysis of organic phosphate esters to alcohol and phosphate ion in an ALKALINE pH
A
ALP
5
Q
TRUE OR FALSE: BONE ISOENZYMES ARE INCREASED IN CHILDREN AND GERIATRIC
A
TRUE. THIS IS DUE TO INCREASED OSTEOBLASTIC ACTIVITY
6
Q
THE LIVER AND BONE ISOENZYMES ARE INHIBITED BY WHAT?
A
LEVAMISOLE AND UREA
7
Q
THE PLACENTA AND INTESTINAL ISOENZYMES OF ALP ARE INHIBITED BY WHAT
A
PHENYLALANINE
8
Q
WHAT CAUSES INCREASED LIVER ALP
A
OBSTRUCTIVE JAUNDICE
9
Q
WHAT CAUSES INCREASED BONE ALP AND THIS HAS THE HIGHEST ELEVATION OF ALP
A
OSTEITIS DEFORMANS OR PAGET’S DISEASE
10
Q
PLACENTAL ALP IS SEEN IN WHAT WEEK OF NORMAL PREGNANCY
A
16-20TH WEEK
11
Q
INTESTINAL ALP IS INCREASED DUE TO INTAKE OF FATTY MEALS. WHAT BLOOD TYPE CAN THIS BE OBSERVED
A
B AND O
12
Q
IN ISOENZYME DIFFERENTIATION FOR ALP, ARRANGE THEM FROM CATHODAL TO ANODAL
A
INTESTINAL –> PLACENTA –> BONE –> LIVER
13
Q
AT WHAT TEMPERATURE AND TIME IS HEAT FRACTIONATION TEST FOR ALP ISOENZYME DIFFERENTITATION SET?
A
56 C FOR 15 MINUTES
14
Q
ARRANGE THE ISOENZYMES OF ALP FROM STABLE TO LABILE
A
PLACENTA –> INTESTINE –> LIVER –> BONE
15
Q
IN UREA INHIBITION TEST FOR ALP ISOENZYME DIFFERENTIATION, WHAT IS INACTIVATED OR DESTROYED WITH THE USE OF 2-3M UREA?
A
BONE AND LIVER ISOENZYME
16
Q
WHAT ARE THE MINOR ISOENZYMES OF ALP
A
REGAN (LUNG) AND NAGAO (PANCREASE)
17
Q
WHAT INHIBITS REGAN ALP?
A
PHENULALANINE
18
Q
WHAT INHIBITS NAGAO?
A
PHENYLALANINE AND L-LEUCINE
19
Q
WHAT ISOENZYME IS MOST STABLE THAN THE PLACENTA ALP
A
REGAN (LUNG)
20
Q
IT IS A VARIANT OF REGAN ALP
A
NAGAO (PANCREAS) ALP
21
Q
IN SPECIMEN CONSIDERATION FOR ALP, ROOM TEMPERATURE CAUSE FALSE DECREASE OF ALP DUE TO WHAT CAUSE?
A
DUE TO LOSS OF CO2
22
Q
THIS IS THE REFERENCE METHOD FOR ALP DETERMINATION
A
BOWER’S AND MCCOMB METHOD
23
Q
THIS IS THE MODIFICATION OF BOWERS AND MCCOMB BY IFCC
A
SZASZ METHOD
24
Q
WHAT IS THE PH AND WAVELENTH OF BOWERS AND MCCOMB METHOD?
A
PH- 10.15
WAVELENTH: 405NM
25
WHAT IS THE SUBSTRATE AND ENDPRODUCT OF BOWERS AND MCCOMB METHOD
SUBSTRATE: P-NITROPHENYLPHOSPHATE
PRODUCT: P-NITROPPHENOL YELLOW NITROPHENOXIDE ION
26
WHAT ARE THE INHIBITORS AND ACTIVATORS FOR ALP?
INHIBITOR: P
ACTIVATOR: Mg Mn
27
WHAT IS THE ENZYME NOMENCLATURE FOR ACP
3.1.3.2
28
WHAT IS THE MAJOR TISSUE FOR ACP
PROSTATE
29
WHAT ARE THE NONPROSTATIC SOURCES FOR ACP?
BONE MARROW, RBC, PLATELETS, LIVER, KIDNEY, SPLEEN, SEMEN
30
OTHER NAME FOR ACP
ACID ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE
31
THIS IS INVOLVED IN THE HYDROLYSIS OF ORGANIC PHOSPHATE ESTERS AND PRODUCES ALCOHOL AND PHOSPHATE ION IN AN ACIDIC PH
ACP
32
VAGINAL WASHING IS EXAMINED FOR SEMINAL FLUID ACP-ACTIVITY AND THIS ENZYMATIC ACTIVITY CAN PERSIST FOR UP TO __ DAYS
4
33
VAGINAL ACP VALUE THAT INDICATES PRESENCE OF SEMINAL FLUID
>50 IU/L
34
IN THE DETERMINATION OF PROSTATIC CARCINOMA: WHAT OTHER TEST IS MORE SENSITIVE THAT IS COMBINED WITH ACP?
PROSTATE SPECIFIC ANTIGEN
35
INCREASED ACP LEVELS ARE OBSERVED IN WHAT DIASEASES?
OSTEOPOROSIS, MULTIPLE MYELOMA, PAGETS DISEASE, GAUCHERS DISEASE, NIEMANN PICH DISEASE, THROMBOCYTOPENIA
36
DECREASED ACP
NOT OF CLINICAL SIGNIFICANCE
37
WHAT DOES HEMOLYSIS DO IN ACP DETERMINATION
FALSE INCREASE
38
THIS CAUSE FALSE DECREASE OF ACP DETERMINATION
USE OF FLUORIDE ANTICOAGULANTS
39
SERUM ACP ____ WITHIN 1-2 HOURS IF LEFT AT ____
FALSELY DECREASES : ROOM TEMP
40
WHAT ARE THE COMMON SUBSTRATES USED IN ALP DETERMINATION?
PARANITROPHENYL PHOSPHATE
THYMOLPHTHALEIN MONOPHOSPHATE
ALPHA-NAPTHYLPHOSPHATE
41
WHAT ARE THE COMMON TESTS FOR ALP?
ROY AND HILLMAN WHICH USES THYMOLPHTHALEIN MONOPHOSPHATE TO FREE THYMPLPHTHALEIN
RIETZ AND GUIBALT WHICH USES 4-METHYLUMBELLIFERYLONEPHOSPHATE TP FLUORESCENCE
42
WHAT ANTICOAGULANT, PH, AND WAVELENTH IS USED FOR ROY AND HILLMAN AND
CITRATE BUFFER | PH 6.0 | 410-450NM
43
THIS ISOENZYME DIFFERENTITATION FOR ACP IS BULKY IN NATURE AND NON-SPECIFIC
CHEMICAL INACTIVATION
44
THIS ISOENZYME DIFFERENTIATION FOR ACP IS NOT EASILY REPRODUCED
ELECTROPHORESIS
45
THIS ISOENZYME DIFFERENTITAION FOR ACP IS RECOMMENDED APPROACH FOR ACP DETERMINATION
IMMUNOASSAY
46
TRAP
RBC ACP
47
NONTRAP
PROSTATIC ACP
48
WHAT INHIBITS RBC ACP
COPPER AND FORMALDEHYDE
49
WHAT INHIBITS PROSTATIC ACP
TARTRATE SINCE IT IS NON-TRAP
50
FORMULA FOR PROSTATIC ACP
TOTAL ACP - TRAP
51
WHAT IS THE ENZYME NOMENCALTURE FOR AST
2.6.1.1
52
MAJOR TISSUES OF AST?
CARDIAC, LIVER, SKELETAL MUSCLE
53
OTHER NAME FOR AST
SGOT SERUM GLUTAMIX-OXALOACETIC TRANSAMINASE
54
MAJOR ISOENZYMES FOR AST
CYTOPLASMIC 80% AND MITOCHONDRIAL 20%
55
WHAT CAUSES AN INCREASE AST
MYOCARDIAL INFARCTION, HEPATOCELLULAR DISCORDER, SKELETAL MUSCLE, CHRONIC LIVER DISORDER, MUSCULAR DYSTROPHY, ACUTE PANCREATITTIS
56
HIGHEST ELEVATIONS OF AST
ACUTE HEPATITIS
57
WHAT CAUSES FALSE ELEVATIONS OF AST
HEMOLYSIS (10X ELEVATIONS), LIPEMIC,A ND ECTERIC
58
WHAT CAUSES DECREASED AST?
UREMIA
59
WHAT IS THE REFERENCE METHOD FOR DETERINING AST?
KARMEN METHOD WHERE WE DETEMINE THE DECREASE IN ABSORBANCE
60
WHAT COUPLED ENZYMES ARE USED IN KARMEN METHOD FOR AST?
NADH AND MALATE DEHYDROGENASE
61
WHAT PH AND WAVELENGTH IS USED FOR KARMEN METHOD
PH 7.5 | 340NM
62
WHAT OTHER METHODS ARE USED FOR AST DETERMINATION ASIDE FROM KARMEN METHOD
REITMAN AND FRANKEL| DIAZONIUM SALT COUPLING| BABSON METHOD
63
WHAT IS THE ENZYME NOMENCALTURE FOR ALP
2.6.1.2
64
MAJOR TISSUE SOURCE FOR ALP
LIVER INTESTINE PLACENTA BONE
65
WHAT ENZYMES DO NOT CONTAIN ANY ISOENZYMES?
ALP, LIPASE, GGT, 5N'T
66
OTHER NAME FOR ALP
SGPT SERUM GLUTAMIC PYRUVIC TRANSAMINASE
67
TRUE OR FALSE: DECREASE IN ALP IS NOT SIGNIFICANT
TRUE
68
WHAT IS THE REFERENCE METHOD FOR ALP DETERMINATION?
COUPLED ENZYMATIC REACTION
69
WHAT COUPLED ENZYMES ARE USED FOR THE DETERMINATION OF ALT
NADH AND LD OR LACTATE DEHYDROGENASE
70
WHAT PH AND WAVELENGTH IS USED FOR COUPLED ENZYMATIC METHOD FOR ALP DETERMINATION
7PH 7.5| 340NM
71
WHAT OTHER METHOD IS USED FOR ALP DETERMINATION ASIDE FROM COUPLED ENZYMATIC REACTION?
DIAZONIUM SALT COUPLING
72
WHAT COENZYME IS USED FOR AST AND ALT DETERMINATION
PYROXIDAL PHOSPHATE VITB6
73
AMINOTRANSFERASES ARE PRESENT IN WHAT SPECIMESNS?
PLASMA, BILE, CSF, SALIVA
74
CHILDREN: AST__ALT
>
75
ADULT: AST __ ALT
76
WHICH HAS A DIURNAL VARIATION AST OR ALT
ALT
77
WHAT IS THE MAJOR ORGAN AFFECTED FOR AST ? FOR ALT?
AST: HEART
ALT: LIVER
78
WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?
ALT: ALANINE ALPHA KETOGLUTARIC ACID
AST: ASPARTATE ALPHA KETOGLUTARIC ACID
79
WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?
AST: GLUTAMIC ACID AND OXALOACETIC ACID
ALT: GLUTAMIC ACID AND PYRUVIC ACID
80
WHAT COLOR DEVELOPER IS USED FOR AST AND ALT?
2-4 DNPH DINITROPHENYLHYDRAZINE
81
WHAT COLOR INTENSIFIER IS USED FOR ALT AND AST?
NAOH
82
BOTH AST AND ALT ARE AFFECTED BY HEMOLYSIS TRUE OR FALSE?
TRUE
83
BOTH AST AND ALT ARE ELEVATED IN HEPARIN THERAPHY 3X
TRUE
84
AT WHAT DISEASE DO BOTH ALT AND AST HAVE ARE DECREASED?
RENAL DISEASE
85
WHAT RATIO IS USED FOR AST AND ALT
DE RITIS RATIO
86
WHAT CAUSES 20X INCREASE OF THE DE RITIS RATIO?
VIRAL AND TOXIC HEPATITIS
87
WHAT DISEASE IS ASSOCIATED WITH HIGHEST ELEVATION OF DERITIS RATIO
ACUTE HEPATITIS
88
WHAT DISEASE IS ASSOCIATED WITH MODERATE ELEVATIONS OF DE RITIS RATIO
CHRONIC HEPATITIS, HEPATIC CARCINOMA, IM
89
WHAT DISEASE IS ASSOCIATED WITH SLIGHT ELEVATIONS OF DE RITIS RATIO?
HEPATIC CIRRHOSIS, ALCOHOL HEPATITIS, AND OBSTRUCTIVE JAUNDICE
90
>2 DE RITIS RATIO
ALCOHOLIC HEPATITIS AND HEPATOCELLULAR DISORDER
91
1-2 DERITIS RATIO
ACUTE HEPATITIS AND CIRRHOSIS
92
<1 DERITIS RATIO
VIRAL HEPATITIS
93
WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE
3.2.1.1
94
MAJOR TISSUE SOURCES OF AMYLASE
PANCREAS AND SALIVARY CELLS OR GLANDS
95
WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE
ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE
96
WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE
ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE
97
OTHER NAME FOR AMYLASE
4-1 GLUCAN-4-GLUCOHYDROLASE
98
IN AMYLASE, PHYSIOLOGIC DIGESTION OF __ AND __ IS OBSERVED
STARCH AND GLYCOGEN
99
THIS IS CONSIDERED AS THE SMALLEST ENZYME
AMYLASE
100
TRUE OF FALSE: AMYLASE IS 15% HIGHER IN FEMALES
TRUE
101
THIS IS THE MOST PREDOMINANT PANCREATIC AMYLASE ISOENZYME IN THE ONSET OF ACUTE PANCREATITIS
P3 ISOENZYME
102
WHAT IS THE OTHER NAME FOR AMS SALIVARY TYPE
PTYALIN
103
WHERE DOES PTYALIN MIGRATE IN ELECTRODE
ANODAL
104
PATHOLOGIC INCREASE OF THE SALIVARY AMYLASE
PAROTITIS
105
OTHER NAME FOR THE PANCREATI AMYLASE
AMYLOPSIN
106
MIGRATION OF PANCREATIC AMYLASE IN THE ELECTRODE
CATHODAL
107
PATHOLOGICAL INCREASE OF PANCRATIC AMYLASE
PANCREATITIS
108
WHAT IS ADDED TO THE SPECIMEN TO AVOID THE INACTIVATION OF THE AMYLASE?
DILUTION WITH NACL
109
WHAT ENDOGENOUS INHIBITORS ARE FOUND IN THE SERUM THAT INHIBIT AMYLASE
WHEAT GERM LECTIN AND TAG
110
WHAT ANTICOAGULANTS INTERFERE WITH AMYLASE
CITRATE, EDTA, OXALATE
111
WHAT SUBSTRATE IS USED IN AMYLASE DETERMINATION
STARCH
112
this test for amylase measures the reducing sugar produced form hydrolyzed starch
SACCHAROGENIC
113
THIS TEST FOR AMYLASE MEASURES THE DRECREASE IN SUBSTRATE CONNCENTRATION WHERE THE TIME OF DECOLORIZATION = THE AMOUNT OF AMYLASE ANCITVITY
AMYLOCLASTIC
114
THIS TEST FOR AMYLASE MEASURES THE INCREASE IN COLOR INTENSITY WHERE THE AMOUNT OF STARCH DYE DESTROTYED = INTENSITY OF COLOR = AMOUNT OF AMYLASE ACTIVITY
CHROMOGENIC
115
THIS METHOD OF AMYLASE DETERMINATION USES A-GLUCOSIDASE AND HEXOKINASE
COUPLED ENZYME
116
THERE ARE 6 BANDS FORMED IN THE ELECTROPHORESIS OF AMYLASE: WHAT ARE THE FIRST 3 BANDS AND THE LAST 3 BANDS?
FIRST: PTYALIN
LAST: AMYLOPSIN
117
THE INHIBITION TEST FOR AMYLASE USES TRITICUM VULGARIS AND IT INHIBITS WHAT TYPE OF AMYLASE
S TYPE OR SALIVARY TYPE OR PTYALIN
118
WHAT IS THE FORMULA FOR AMYLASE CREATININE RATIO:
URINE AMYLASE/ SERUM AMYASE X SERUM CREATININE/ URINE CREATININE
119
WHAT IS THE NORMAL REF RANGE FOR AMYLASE CREATININE RATIO?
1-5%
120
INCREASED AMYLASE IN BLOOD AND URINARY SECRETIONS INDICATE WHAT DISEASE. AC RATIO IS 4-15%
ACUTE PANCREATITIS
121
WHAT DISEASE CAUSES ELEVATED AMYLASE IN THE SERUM AND NORMAL AMYLASE URINARY SECRETION? THIS IS CHARACTERIZED BY AMYLASE BOUNDED TO AN ANTIBODY
MACROAMYLASEMIA
122
WHAT IS THE ENZYMATIC NOMENCLATURE FOR LIPASE
3.1.1.3
123
MAJOR TISSUE SOURCE OF LIPASE
PANCREAS
124
ANOTHER NAME FOR LIPASE
TRIAGLYCEROL ACYLHYDROLASE
125
IT INVOLVES THE HYDROLYSIS OF FATS TO PRODUCE ALCOHOL AND FATTY ACIDS
LIPASE
126
THIS IS THE SUPERIOR DIAGNOSTIC TOOL FOR PANCREATITIS
LIPASE
127
THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE
ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS
128
THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE
ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS
129
DECREASED LIPASE VALUES ARE OBSERVED IN
PANCREATIC INSUFFICIENCY
130
WHAT SUBSTRATE IS USED FOR LIPASE MEASUREMENT
OLIVE OIL
131
WHAT IS AN ALTERNATIVE FOR THE SUBSTRATE IN LIPASE MEASUREMENT
TIOLEIN OIL
132
THIS INCREASES THE SENSITIVITY AND SPECIFICITY OF LIPASE MEASUREMENT
BILE SALTS AND COLIPASE
133
THIS PREVENTS LIPASE INACTIVAITON
134
THIS PREVENTS LIPASE INACTIVATION
COLIPASE
135
INHIBITOR FOR LIPASE AND WHICH CAUSES FALSE DECREASE OF LIPASE
HEMGLOBIN (HEMOLYSIS)
136
BACTERIAL CONTAMINATION CAUSES __ IN LIPASE VALUES
FALSE INCREASE
137
WHAT IS THE REFERENCE METHOD FOR LIPASE DETERMINATION
CHERRY CRANDAL METHOD
138
THIS USES OLIVE OIL IN THE DETERMINATION OF LIPASE. INCUBATIION IS DONE FOR 24 HOURS AT 37 C
CHERRY CRANDAL METHOD
139
THIS IS COMMONLY USED METHOD FOR LIPASE MEASUREMENT; IT IS A COLORIMETRIC METHOD THAT USES MINIMAL SAMPLE
peroxidase coupling
140
THIS LIPASE MEASUREMENT USES BOTH OLIVE AND TIROLEIN OIL
TURBIDIMETRY
141
TRUE OR FALSE: DECREASE IN TURBIDIMETRY INDICATE DECREASE IN LIPASE?
FALSE. DECREASE IN TURBIDIMETRY = INCREASE LIPASE
142
WHAT IS THE ENZYMATIC NOMENCLATURE FOR LACTATE DEHYDROGENASE?
1.1.1.27
143
THIS ENZYME CAUSES THE INTERCONVERSION OF LACTIC AND PYRUVIC ACID
LACTATE DEHYDROGENASE
144
WHAT COENZYME IS USED IN LACTATE DEHYDROGENASE?
NAD NICOTINAMIDE ADENINE DINUCLEOTIDE
145
WHAT ARE THE 2 FORMS OF LACTATE DEHYDROGENASE
H AND M FORMS
146
WHAT ARE THE MAJOR ISOENZYMES OF LACTATE DEHYDROGENASE
LD1, 2, 3, 4, 5
147
WHAT IS THE MINOR ISOENZYME OF LACTATE DEHYDROGENASE
LD6
148
WHAT LD ISOEZNYME HAS THE HIGHEST PERCENTAGE
LD2
149
what are the sources of LD1 and LD2 clue: RK heart
RBC KIDNEY AND HEART
150
WHAT ISOENZYME OF LD IS THE MOST ANODAL AND HEAT STABLE
LD 1 AND 2
151
WHAT ARE THE SOURCES OF LD 2 (CLUE PLS)
PANCREAS LUNGS SPLEEN
152
THE IS THE INTERMEDIARY ISOENZYME. MODERATE IN ABUNDANCE
LD3
153
WHAT ARE THE SOURCES OF LD 4 AND 5 (CLUE SSmILI)
SKIN, SMALL, ILIUM, LIVER, INSTESTINE
154
THIS IS THE LEAST ANODAL AND AND HEAT LABILE AND ARE SCARCE
LD 4 AND LD 5
155
WHAT IS ANOTHER NAME FOR LD 6
ALCOHOL DEHYDROGENASE
156
AT WHAT BAND DOES THE LD6 APPEAR IN ELECTROPHORESIS
6TH BAND
157
THIS IS OBSERVED WHEN THERE IS HIGHEST ELEVATIONS OF LACTATE DEHYDROGENASE
PERNICIOUS ANEMIA
158
NORMAL ELECTROPHORETIC PATTERN OF SERUM LD AS WELL AS WHEN THERE IS SEIZURE
2>1>3>4>5
159
CSF AND ACUTE MYOCARDIAL INFARCTION LD ELECTROPHORETIC PATTERN
1>2>3>4>5
160
LD ELETROPHORETIC PATTERN FOR BACTERIAL MENINGITIS
5>4>3>2>1
161
WHAT LD ISOENZYMES ARE CONSIDERED AS CANCER MARKERS.
LD 2 3 4
162
WHAT LD IS ASSOCIATED WTH HEPATIC CARCINOMA ANND TOXIC HEPATITIS
ld 5
163
THIS ENZYME IS SEVERELY AFFECTED BY HEMOLYSIS
LD LACTATE DEHYDROGENASE
164
LD LEVEL (>/
>
165
TRUE OR FALSE: THERE IS DECREASE VALUE OF LD IF SAMPLE IS FROZEN
TRUE. THE STORAGE FOR LD IS AT ROOM TEMP 20 TO 26 C
166
THIS METHOD FOR LD CONVERTS LACTATE TO PYRUVATE AT PH 8.8 AND THIS IDENTIFIES LD 1
WACKER METHOD
167
THIS LD METHOD CONVERTS PYRUVATE TO LACTATE AT PH 7.2 AND IDENTIFIES LD 5
WROBLEWSKI LADUE (REVERSE METHOD
168
IN HEAT DENATURATION FOR LD MEHTODS WHAT ISOENZYME IS STABLE
LD 1
169
WHAT IS THE ISOENZYME NOMENCLATURE FOR CREATINE KINASE
2.7.3.2
170
MAJOR TISSUE SOURCES FOR CREATINE KINASE
CARDIAC MUSCLE, SMOOTH MUSCLE, SKELETAL MUSCLE AND BRAIN
171
TRUE OR FALSE: ATHLETES HAVE INCREASE BASELINE LEVEL OF CREATINE KINASE
TRUE
172
THIS CAUSES INCREASE OF CK LEVELS FOR UPTO 5 TIMES
INTRAMUSCULAR INJECTION
173
TRUE OR FALSE: BEDRIDDEN PATIENTS HAVE DECREASED CK VALUES
TRUE
174
THIS IS A MINOR ISOENZYME OF CK SEEN IN ADVANCED MALIGNANCIES
MITOCHONDRIAL CK
175
WHAT ARE THE DIFFERENT ISOZENZYMES OF CK
CK-BB CK-MB AND CK-MM
176
THE MOST ANDODAL AND MOST LABILE CK ISOENZYME
CK BB
177
HOW MANY PERCENT OF CK-MB IS PRESENT IN CARDIAC TISSUE?
| WHAT PERCENT OF TOTAL CK INDICATES MYOCARDIAL DAMAGE
20% OF CK MBB IS PRESENT IN CARDIAC TISSUE
| >6% OF TOTAL CK = MYOCARDIAL DAMAGAE
178
THIS IS THE MAJOR ISOENZYME OF CK IN THE SERUM
CK-MM
179
THIS IS THE LEAST ANODAL CK ISOENZYME
CK-MM
180
HIGHEST ELEVATIONS OF CK IS SEEN IN THIS DISEASE
DUCHENE DISORDER
181
PERCENTAGE OF TOTAL CK ATTRIBUTED TO CK-MB
| POSSIBLE RELEASE OF CK-MB FROM NON-CARDIAC TISSUE
CK RELATIVE INDEX
182
CK ACTIVATOR
N-ACETYL-CYSTEINE AND MG
183
WHAT SUBSTANCE IS FOUND IN THE RBC CAUSING INCREASED CK
ADENYLATE KINASE
184
INHIBITOR OF CK
URATE AND CYSTEINE
185
TRUE OR FALSE: CK IS PHOTOSENSITIVE
TRUE
186
CK WOULD BE DECREASED DURING STORAGE, WHAT REAGENTS ARE USED TO REMEDY THIS
CLELAND'S REAGENT AND GLUTATHIONE
187
THIS IS THE FORWARD METHOD IN DETECTING CK. WHAT PH AND WAVELENGTH IS USED?
TANZER-GILOVARD METHOD | pH 9 | 340 nm
188
THIS IS THE INDIRECT METHOD IN MEASURING CK. WHAT PH AND WAVELENTH IS USED?
OLIVER-ROSALSKI METHOD
PH6.8
340NM
189
WHAT IS THE ENZYME NOMENCLATURE FOR GGT
2.3.2.1
190
THIS ENZYME IS AN INDICATOR FOR ALCOHOLISM
GGT
191
HIGHEST ELEVATIONS OF GGT
PRIMARY BILIARY CIRRHOSIS
192
WHAT METHOD IS USED FOR GGT DETERMINATION AND WHAT WAVELENGTH IS USED?
SZASZ ASSAY
| 405-410 nm
193
WHAT IS THE ENZYME NOMENCLATURE FOR TRUE AND PSEUDO CHOLINESTERASE
3. 1.1.7 TRUE CHOLINESTERASE
| 3. 1.1.8 PSEUDOCHOLINESTERASE
194
WHAT IS ANOTHER NAME FOR TRUE CHOLINESTERASE
ACYLCHOLINE ACYLHYDROLASE
195
WHAT IS ANOTHER NAEM FOR PSEUDOCHOLINESTERASE
ACETYLCHOLINE ACETYLHYDROLASE
196
THIS ENZYME IS USED IN THE ASSESSMENT OF PARENCHYMAL FUNCTION
CHOLINESTERASE
197
INHIBITOR FOR CHOLINESTERASE
SUCCINYLCHOLINE
198
WHAT IS THE MOST USED METHOD IN CHOLINESTERASE? WHAT WAVELENGTH IS USED?
ELLMAN
| 410 NM
199
THIS METHOD IN CHOLINESTERASE DETERMINATION MAKE USE OF A SPECIALIZED EQUIPMENT
MICHEL
200
WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE
4.1.2.13
201
WHAT IS OTHER NAME FOR ALDOLASE
FRUCTOSE 1-6 DIPHOSPHATE ALDOLASE
202
ISOENZYMES OF ALDOLASE AND WHERE ARE THEY FOUND?
ALDOLASE A: SKELETAL MUSCLE
ALDOLASE B : WBC, LIVER, KIDNEY
ALDOLASE C: BRAIN TISSUE
203
WHAT IS THE MAIN TISSUE SOURCE OF OCT
LIVER
204
WHAT METHOD IS USED FOR OCT
REICHARD AND REICHARD
205
WHAT IS THE ENZYMATIC NOMENCLATURE FOR OCT
2.1.1.3
206
WHAT ARE THE MAIN SOURCES OF LEUCINE AMINOPEPTIDASE
BUS
| BILE URINE SERUM
207
WHAT IS THE METHOD FOR LEUCINE AMINOPEPTIDASE
GOLDBERG AND RUTENBERG
208
WHAT ARE THE LABORATORY METHODS FOR 5NT
DIXON AND PURDON
BELLFIELD AND GLODBERG
CAMPBELL
209
ACE NOMENCLATURE
3.4.15.1
210
MAIN SOURCE OF ACE
MACROPHATGES AND EPITHELOID CELLS
211
THIS ENZYME IS INCREASED IN ALZHEIMERS DISEASE IN VIA CSF ANALYSIS
ACE
212
CERULOPLASMIN NOMENCLATURE
1.16.3.1
213
ENZYME THAT IS A KNOWN MARKER FOR WILSONS DISEASE
CERULOPLASMIN
214
THIS ENZYME MAINTAINS THE NADPH IN A REDUCED FROM INSIDE THE RBC
G6PD
215
G6PD NOMENCLATURE
1.1.1.49
