Flashcards in Epigenetics Deck (11):
3 ways to end up with cardiac hypertrophy
1. High BP
2. Heart Attack
3. Genetic mutation
How were mice used for models of cardiac hypertrophy?
Induced hypertrophy via pressure overload in mice by creating a Transverse Aortic Constriction (surgery)
Hallmarks of Cardiac Hypertrophy
i. New protein synthesis
ii. Sarcomere assembly
iii. Gene Expression
Definition of epigenetics
Reversible markers that can alter gene transcription without changing the underlying nucleotide sequence
Most commonly modified AA on histone tails
How does ChIP work?
1. Isolate with formin
3. Use Ab coupled with PCR to immunoprecipitate out desired sequence. The chromatin that is acetylated gets pulled down w/ Ab
HAT vs HDAC
HAT = writer = activates gene
HDAC = eraser = deactivates gene
How can you study protein function?
Gain of function via Transgenic overexpression
Loss of function via KO and chemical inhibition
HDAC9 does what to cardiac hypertrophy? How was it demonstrated?
Repressor of cardiac hypertrophy
Shown via HDAC9 knockdown leading to hypertrophy
How do you block HDAC9?
Use small interfering RNA (siRNA) to block --> inhibits HDAC9 protein --> with added stress (TAC)= cardiomyocyte hypertrophy