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Flashcards in Epigenetics Deck (11):
1

3 ways to end up with cardiac hypertrophy

1. High BP
2. Heart Attack
3. Genetic mutation

2

How were mice used for models of cardiac hypertrophy?

Induced hypertrophy via pressure overload in mice by creating a Transverse Aortic Constriction (surgery)

3

Hallmarks of Cardiac Hypertrophy

i. New protein synthesis
ii. Sarcomere assembly
iii. Gene Expression

4

Definition of epigenetics

Reversible markers that can alter gene transcription without changing the underlying nucleotide sequence

5

Most commonly modified AA on histone tails

Lysine

6

How does ChIP work?

1. Isolate with formin
2. Fragment
3. Use Ab coupled with PCR to immunoprecipitate out desired sequence. The chromatin that is acetylated gets pulled down w/ Ab

7

HAT vs HDAC

HAT = writer = activates gene
HDAC = eraser = deactivates gene

8

How can you study protein function?

Gain of function via Transgenic overexpression
OR
Loss of function via KO and chemical inhibition

9

HDAC9 does what to cardiac hypertrophy? How was it demonstrated?

Repressor of cardiac hypertrophy

Shown via HDAC9 knockdown leading to hypertrophy

10

How do you block HDAC9?

Use small interfering RNA (siRNA) to block --> inhibits HDAC9 protein --> with added stress (TAC)= cardiomyocyte hypertrophy

11

Overexpression of HDAC9 =?

block cardiomyocyte hypertrophy