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formula of retic count

# of retics / 1000 RBCs observed x 100

1

formula for retics in Miller disc

# of retics x 100 / # of RBCs x 9

2

other name of Wright-Giemsa stain

Romanowsky stain

3

this is a polychrome stain

Wright-Giemsa stain

4

formula for diluted ESR

(undil. ESR x .86) - 12

5

first stage of ESR

lag phase

6

duration of stage 1 ESR

10mins

7

duration of stage 2 ESR

40mins

8

stage 2 of ESR

decantation or rapid/fast phase

9

stage 3 of ESR

slow sedimentation

10

duration of stage 3

10mins

11

causes of increased ESR

osteomyelitis
stroke
pregnancy
multiple myeloma
hyperfibrinogenemia

12

cause of decreased ESR

sicke cell anemia

13

length of Westergren

30cm

14

length of Wintrobe

11.5cm

15

bore diameter of Westergren

2.5mm

16

bore diameter of Wintrobe

3mm

17

tube whose bottom is open

Westergren

18

tube whose bottom is flat/close

Wintrobe

19

anticoag of blood used for Westergren

citrate

20

anticoag of blood used in Wintrobe

double oxalate or Paul Heller

21

disadvantage of Westergren

large amount of blood needed

22

advantage of Wintrobe

simple, no dilution

23

this method spins tubes in a vertical position in 4-45second cycle for 3mins

zetafuge

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duration of zetafuge

3mins

25

cycle of zetafuge

4-45 second cycle

26

formula of zetcrit

true hct / ZCT

27

dilution for RBC count

1/200

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dilution for WBC count

1/20

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depth of counting chamber

1/10 mm

30

size of primary square

9sq.mm

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size of secondary square

1x1mm

32

size of tertiary squares

.0625sq.mm

33

size of the smallestsquares

.0025sq.mm

34

depth of Fuchs-Rosenthal

.2mm

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size of primary square in Speirs-Levy

10sq.mm

36

corrective measure of RBC count of anemic blood

RBC count x 5000
or
divide result by 2
or
decrease dilution (blood up to 1 mark)

37

formula for corrected WBCs

uncorrected WBC x 100 / # of NRBCs per 100 WBCs + 100

38

microscope for platelet count

phase-contrast microscope

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color o platelet count in phase contrast

light purple sheen

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platelets gather around neutrophils

platelet satellitosis

41

component of Drabkin

potassium ferricyanide
potassium cyanide
sodium bicarbonate
surfactant

42

what converts Hb to metHb

potassium ferricyanide

43

what converts metHb to cyanmetHb

potassium cyanide

44

at what absorbance is cyanmethemoblobin read?

540nm

45

sensitive to light

cyanmetHb

46

how long does it take carboxyHb to convert to metHb

1hr

47

used to convert Hb to SLS-Hb

sodium lauryl sulfate

48

normal value for MCV

80-100

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normal value for MCHC

32-36

50

value to report microcytic

<80

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value to report macrocytic

>100

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value to report as hypochromic

<32

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macrocytic, normochromic is found in

liverdisease, myelodysplasia, megaloblastic anemia

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microcytic, hypochromic is found in

IDA, thalassemia, sideroblastic anemia

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normocytic, normochromic is found in

hemolytic anemia, leukemia, chronic renal disease

56

average cocentration of hemoglobin in each individual erythrocyte

MCHC

57

last inmature erythrocyte stage

reticulocyte

58

used to assess the erythropoietic activity of the bone marrow

retic count

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stain for retic count

supravital stain

60

in miller disc, what are counted in the smaller square

RBCs

61

In miller disc, what are counted in the bigger square

retics

62

formula for percent retic

# of retic in square A / # of RBCs in square B

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minimum # of cells counted in small square

112 cells

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represents precipitated Hb

Heinz bodies

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round nuclear fragments and are usually singular

Howell-Jolly

66

hemosiderin in mitochondria, stained with prussian blue

Pappenheimer bodies

67

formula for absolute retic count

percent retic x RBC count / 100

68

normal values for ARC

25-75 x 10^9

69

formula for corrected retic count

% retic x (patient's Hct / 45)

70

retics released from the marrow prematurely are called

shift retics

71

used to determine appropriate corection factor for RPI

patient's Hct

72

correction factor when patient's Hct is 40-45

1 (in days)

73

correction factor when patient's Hct is 35-39

1.5

74

correction factor when patient's Hct is 25-34

2

75

correction factor when patient's Hct is 15-24

2.5

76

correction factor when patient's Hct is < 15

3

77

formula for RPI

(retic % x (Hct/45)) / maturation time

78

distance in millimeters that the RBCs fall in 1hr

ESR

79

RBC aggregate are called

rouleaux

80

ESR is directly proportional to

RBC mass

81

ESR is inversely proportional to

plasma viscosity

82

this is diagnostic testing at or near the site of patient care

point-of-care testing

83

requirements of point-of-care testing

POC testing coordinator
written policy
ongoing evaluation

84

plasma, in terms of ekectrical current is a

conductor

85

alternate formula for RPI

corrected retic count / maturation time

86

what complex stains neutral components in a blood film

thiazine-eosinate

87

ph of buffer for staining

6.4

88

buffer used for staining

.05M sodium phosphate

89

presence of fibrin stains would

render the film useless