Eukaryotic genomes

Question 1

Why do we sequence genomic DNA?

Answer 1

proteins are difficult to store
they cannot be modified and preserved
the mechanism of expression is difficult to demonstrate

Question 2

What are the advantages of sequencing?

Answer 2

DNA is more stable and easier to store
genes can be kept for studies in bacterial plasmids
the genetic sequence can be expressed in bacteria or in vitro systems in large quantities and high purity
genes can be introduced into cells by transfection

Question 3

what does a promoter function of the gene do?

Answer 3

regulates the expression of the gene

Question 4

Where are proteins made in the cell?

Answer 4

Cytoplasm

Question 5

how long is the haploid genome?

Answer 5

2.3 billion base pairs long

Question 6

Which was the first multicellular organism that was sequenced?

Answer 6

C.elegans
19,099 genes, 97Mb

Question 7

What are the problems with dispersed DNA repeats

Answer 7

True and repeat overlaps
Contrive and repeat boundaries

Question 8

Why can’t cDNA be sequenced?

Answer 8

cDNA is complimentary DNA which is reversed transcribed from RNA
cDNA is coding regions which does not have introns - only exons
genes are not expressed all in the same cell