Exam 1 Flashcards

1
Q

We wished to determine the abundance of bacteria in a sample of milk. To do this ten-fold serial dilutions of a one milliliter milk sample were prepared. One milliliter of the fourth dilution was plated in a pour plate. After incubation, the plate had 32 colonies. How many bacteria were in the original milk sample?

A

320,000 cells per ml

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2
Q

Which of the following statements is/are correct?
A. Flagella allow bacteria to glide on surfaces
B. Flagella allow bacteria to swim
C. The directed movement of bacterial cells in response to a gradient of a chemical, is called chemotaxis

A

Flagella allow bacteria to swim and The directed movement of bacterial cells in response to a gradient of a chemical, is called chemotaxis

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3
Q

Bacteriostatic compounds

A

are biological or chemical agents that stop bacteria from reproducting

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4
Q

An isolated colony on a streak plate typically contains millions of cells that are the progeny of a single cell

A

this is a true statement

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5
Q

Toxic hydrogen peroxide produced in some bacteria can be broken down into water and oxygen by which enzyme

A

catalase

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6
Q
Which of the following is NOT likely to be a reason for why exponential growth stops in bacterial cultures grown in a lab?
A. accumulation of waste products
B. exhaustion of nutrients
C. harmful changes in pH
D. Bacteria becoming dormant
A

bacteria becoming dormant

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7
Q

which of the following are required for microbial cell growth?
A. Reduced cofactors
B. anabolic intermediates
C. Energy conserved in chemical bonds.

A

all,
A. Reduced cofactors
B. anabolic intermediates
C. Energy conserved in chemical bonds.

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8
Q

The growth of almost all bacteria in the environment

A

is typically limited by the availability of one or more nutrients

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9
Q

Antoni can Leeuwenhoek developed and used

A

a microscope with a single lens to view bacteria

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10
Q

the cell membrane of bacteria

A

is semi permeable and is usually a lipid bilayer

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11
Q

prokaryotes and eukaryotes

A

have many things in common because they share a common ancestor

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12
Q

Penicillin interferes with bacterial cell growth by

A

inhibiting the formation of shor polypeptide crosslinks between strands of polysaccharide in peptioglycan that re made of repeated units of Nacetylglucosamine (NAG) linked to N-acetylmuramic acid

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13
Q

Bacterial cells reproduce by binary fission, which results in exponential growth of the population. If the size of a bacterial population at time zero (t0) is 20 cells and the generation time is 30 minutes, how many cells will be in the population after 3 hours of growth?

A

1280 cells

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14
Q

There are similarities and differences between gram negative and positive bacteria. Which of the following is not true?
A. The cell wall of Gram-negative cells is composed of only one or two layers of peptidoglycan whereas the cell walls of Gram-positive bacteria have many peptidoglycan layers.
B. Only gram positive bacteria have lipopolysaccharide that extends out the cell surface
C. The cell membranes of both Gram-negative and Gram-positive bacteria are lipid bilayers.
D. There is a second membrane (called the outer membrane) just outside the cell wall of Gram-
negative bacteria, but this is absent from Gram-positive bacteria.

A

Only Gram positive bacterial calls have lipopolysaccharide that extends out the cell surface.

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15
Q

Which of the following statements about phospholipids is true?

a. They are comprised of molecules that are entirely hydrophilic.
b. They are comprised of molecules that are entirely hydrophobic.
c. They are the principle components of bacterial cell membranes.
d. have “heads” and “tails” that both have phosphate groups.

A

They are the principle components of bacterial cell membranes

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16
Q

Approximately two billion years ago, ______ were primarily responsible for initially oxygenating earth

A

cyanobacteria

17
Q

Koch’s postulates

A

are steps that must be followed to determine the cause of an infectious disease

18
Q

gram-positive bacteria

A
  1. have a thick cell wall which retains crystal violet dye
  2. contain teichoic acid in their cell walls
  3. appear purple when gram stained
19
Q

The cell envelope of Gram negative bacteria

A

includes both an inner and outer membrane and includes a peptidoglycan layer

20
Q

Archaea and bacteria are unified as prokaryotes in lacking

A

nuclei and membrane enclosed organelles

21
Q

when a bacterial culture contains only one type of organism it is called a _____

A

pure culture

22
Q

Which of these are routinely used to obtain a pure culture of bacteria

A

agar streak plates

23
Q

pasteurization requires

A

the use of heat to kill most of the microorgamisms present in liquids

24
Q

bacterial growth is usually graphed using ____

A

a semi logarithmic plot

25
Q

most bacteria reproduced by

A

binary fission

26
Q

the lipopolysaccharide (LPS) layer is found ONLY in the cell surface of

A

gram negative bacteria

27
Q

what are environmental factors that influence microbial growth?

A

oxygen, temperature, osmotic pressure, the kinds and concentrations of available nutrient

28
Q

generation time can best be defined as

A

the time it takes for a cell to divide

29
Q

Today the enrichment culture technique developed over a century ago by Martinus Beijerinck remains a feasible approach to obtain bacterial populations that are able to degrade chemicals that are environmental pollutants.

A

True

30
Q

The “plate count anomaly” refers to the fact that the number of bacteria seen in a sample using microscopy is less than the actual number of cells in the sample.

A

False

31
Q

Aseptic techniques are a collection of practices that prevent contamination of cultures.

A

True

32
Q

Not only do some microorganisms tolerate extremely hot temperatures, some actually require high temperatures for optimal growth.

A

Ture

33
Q

The largest mass of living material on Earth comes from microorganisms.

A

True

34
Q

Gelatin is the solidifying agent that is most widely used in bacteriological growth media.

A

False

35
Q

According to our present understanding, each of the three major domains of organisms has what is known as its own universal ancestor.

A

true

36
Q

By using swan-necked flasks Pasteur was able to prove the theory of spontaneous generation.

A

False