EXAM 2 Flashcards
(10 cards)
in centrifugation, which particles are at the bottom
the more dense ones
describe gel filtration chromatography
column is filled with porous beads that have openings that allow only small proteins to flow through, the larger proteins flow around and come out first.
describe ion exchange chromatography
The matrix is either positive or negative, if the matrix is negative the negatively charged proteins will flow through faster with the positive proteins
describe affinity chromatography
the matrix consists of a ligand for a specific protein, the specific protein binds to the matrix while others pass thru, then a buffer solution with a competing ligand is passed through and the specific protein flows through.
what size proteins separated by SDS PAGE is done in low percentage gels
large proteins
what size proteins separated by SDS PAGE is done in high percentage gels
small proteins
where is the proximal histidine located
on the F helix backbone
what does the distal histidine bind to
the O2 that is binded to the heme group
What state is hemoglobin in when it is bound to O2
R state
what state is hemoglobin in when its not bound to O2
T state
