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Flashcards in Exam 2 Deck (44):
1

Which amino acid in the aquaporin channel hydrogen bonds with water that is being transported

Asn

2

The Na K-ATPase is a ______ that moves Na from _____ and K from

Antiporter, inside to out, outside to in

3

Any sugar that has a free aldehyde group is called

A reducing sugar

4

Glycogen contains primarily ______ glycosidic bonds with periodic ______ glycosidic bonds as Branch points

Alpha 1 through 4 and Alpha 1 through 6

5

O linked oligosaccharides are commonly attached to the oxygen of

Serine and threonine

6

N linked oligosaccharides are commonly attached to a sugar amide nitrogen of

Asparagine

7

What is the purpose of the poly a tail on eukaryotic mRNA

It increases the half-life of the MRNA transcript

8

What tend to be water soluble, have a transmembrane, are hydrophobic and hydrophilic

Integral membrane proteins

9

Enzymes that cleave DNA at specific sequences are called

Restriction endonucleases

10

The linkage between monomer nucleotide units in DNA and RNA is a

Phosphodiester Bond

11

The replication of DNA to form daughter strands is

Semi conservative

12

One thing required for DNA replication of a new strand is

A primer

13

TRNA anticodons aligned with the

Codon on mRNA

14

PCR requires a polymerase that is resistant to

Heat

15

DNA sticky ends are joined using the enzyme

Ligase

16

In blue white screening of E coli containing recombinant Lee cloned genes of Interest, What gene on the vector is cut open to insert the gene of Interest, thereby inactivating this Gene

Lac z

17

What changes the supercoiling of DNA

Topoisomerase

18

What separates double-stranded DNA into single stranded DNA in front of DNA polymerase

DNA helicase

19

What do single strand binding proteins do

Cheap DNA in a single strand State and protect single strand DNA from damage

20

DNA that encodes proteins undergoes the process of transcription to produce

MRNA

21

What are the special sequences just Upstream of starting site for RNA polymerization called

Promoter

22

What transport requires no energy consumption in moves from an area of high concentration to an area of low concentration

Passive transport

23

What transport requires energy, transports large molecules and pushes small molecules against a concentration gradient

Active transport

24

What transport is highly selective, gated, and controlled and has a conformational change

Membrane transport

25

No double bonds and saturated with hydrogen's

Saturated fat

26

Contains double bonds and are in a cis confirmation

Unsaturated oil

27

Reaction of OH with acid to produce Ester and water

Esterification

28

What regulates NaCl + H2O transport across epithelial cells plasma membrane in cystic fibrosis

Cftr protein

29

What can't humans digest but termites can

Beta linkages from cellulose

30

What bond has an increased energy a negative Delta G

Phospho anhydride Bond

31

What has an anticodon that complimentary hydrogen bonds with codon of mRNA

tRNA

32

What Cleves and catalyzed hydrolysis of phosphodiester bonds

Nucleases

33

What are the steps to DNA repair

1. Damage recognition
2. Base excision
3. AP site incision
4. DNA repair synthesis ( DNA polymerase)
5. DNA ligation ( ligase)

34

Why doesn't Chargaffs rule hold true for RNA

Because RNA is single stranded and DNA is double-stranded

35

What forces of G-C pairs are stronger than A-T pairs

Stacking forces. It does not depend on the number of hydrogen bonds in the base pairs. Organisms that survive in hot environments contain more GC and have a greater stability of DNA

36

Do you have any issues produce bases that are foreign today night. Making them quickly spotted and repaired before DNA has replicated and damages passed to the Next Generation

DNA contains bases acgt making it easy to repair

37

Why is RNA synthesis less accurate than DNA synthesis

There's a higher rate of error in RNA transcription because the cells survival does not depend on accurately synthesize RNA. If translated, RNA containing error madeley to defective protein which will be destroyed by the cell before it does damage

38

Adenosine derivative cordycepin inhibits RNA synthesis

It can be phosphorylated and used as a substrate by RNA polymerase. It blocks further RNA polymerization because it takes a 3 prime OH group

39

What silence Gene for vascular endothelial growth factor. Addition of Si RNA targets against vascular endothelial growth factor Gene

RNA interference

40

Pairing of Transfer RNA anticodon with messenger RNA codon from five Prime end of codon. Once first two positions are paired, exact base pairing of third position is less critical

Wobble position

41

In _________ translation begins before mRNA transcription has been completely synthesized

Prokaryotes

42

Why is co transcriptional translation not possible in eukaryotes

RNA is produced in the nucleus and ribosomes are located in the cytosol so they are in separate compartments and cannot occur.
Prokaryote in mRNA and protein synthesis takes place in the cytosol so it can take place

43

What are required in Vivo, they prevent interaction and aggregation of other proteins. Assist in protein folding and do not contribute any additional info regarding tertiary structure of the protein. It's contained in the primary structure or native conformation

Chaperones

44

What can yield more than one polypeptide

Single RNA template