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Flashcards in FA Gram Stain Taxonomy Deck (21):
1

What are the gram positive cocci?

Staph and strep

2

What are the gram negative cocci?

Neisseria

3

What are the gram positive rods?

Clostridium
Corynbacterium
Bacillus
Listeria
(Mycobacterium - acid fast)
(Gardnerella - Gram variable)

4

What are the respiratory gram negative rods?

Haemophilus (pleomorphic)
Legionella (silver)
Bordetella

5

What are the zoonotic gram negative rods?

Francisella
Brucella
Pasteurella
Bartonella

6

There are 13 enteric gram negative rods. Tell me 5. In micro, assume gram negative rod unless you have memorized it to be something else.

E coli
Shigella
Salmonella
Yersinia
Klebsiella
(learn rest later I guess)

7

What are the gram positive branching filamentous bacteria?

Actinomyces
Nocardia (weakly acid fast)

8

What are the pleomorphic gram negatives?

Rickettsiae (Giemsa)
Chlamydiae (Giemsa)

9

What are the spiral spirochetes (which I guess you consider to be gram negative...)?

Leptospira
Borrelia (Giemsa)
Treponema

10

What bacteria has a sterol-containing membrane and no cell wall?

Mycoplasma (gram invisible!)

11

What bacteria has a my colic acid containing membrane and high lipid content?

Mycobacteria

12

There are 6 bugs that do not gram stain well. These Rascals May Microscopically Lack Color

Treponema
Rickettsia
Mycobacteria
Mycoplasma
Legionella pneumophila
Chlamydia

13

This bug does not gram stain well because it is too thin to be visualized.

Treponema

14

How do you visualize Treonemes?

Dark-field microscopy and fluorescent antibody staining

15

What intracellular parasite can't be gram stained?

Rickettsia

16

What bacteria can't be gram stained do to the high lipid content of its cell wall?

Mycobacteria

17

How do you detect mycobacteria and why does that work?

high lipid content in cell wall deterred by carbolfuchsin in ACID FAST stain

18

What bacteria can't be detected by gram stain because it has no cell wall?

Mycoplasma

19

Why can't you see Legionella on gram stain?

Primarily intracellular

20

What do you use to visualize Legionella?

Silver stain.

21

Why bacteria can't be seen on gram stain because it is an intracellular parasite that lacks muramic acid in its cell wall?

Chlamydia