Factors Affecting Enzymes

Question 1

Effect of ph on product formation

Answer 1

Active site of enzyme denatures
Fewer enzyme substrate complexes as substrate can’t bind
Reaction incomplete

Question 2

Effect of ph on rate

Answer 2

Two curve left and right - active sits denatures, substrate can’t Bind , products can’t form
Reaction slows
Peak - optimum ph

Question 3

Effect of enzyme concentration on product formation

Answer 3

Higher conc
More enzymes
More enzyme substrate complexes
Product made faster

Question 4

Enzyme concentration effect on rate

Answer 4

Straight direct proportion graph
As enzyme conc increases more enzyme substrate complexes
Faster rate as product made faster
Only limited by amount of substrate

Question 5

Explain competitive inhibitors

Answer 5

Molecules with similar shape to substrate
Bind to active site
Prevent enzyme substrate complexes from forming and original reaction to happen

Question 6

Explain non competitive inhibitors

Answer 6

Molecules attach to binding site on an enzyme that is not the active site
Changes tertiary structure of active site permanently
No more enzyme substrate complexes
No longer complementary