Fermentation Flashcards
what is fermentation?
the anaerobic fate of pyruvate
NADH is a substrate for which reaction and must undergo what for glycolysis to continue?
for the GAPDH reaction, it must be oxidized
In muscle pyruvate is ** to ** to regenerate **
reduced, lactate, NAD+
yeast decarboxylates pyruvate to form ** and ** and it requires which cofactor?
CO2 and ethanol, needs TPP
the fate of pyruvate in the citric acid cycle is
CO2 and H20
detail homolactic fermentation
when pyruvate is converted to L-lactate by lactate dehydrogenase, NADH is oxidized. It occurs when demand for ATP is high and O2 concentration is low. Te lactate produced is taken to the liver to undergo gluconeogenesis
detail LDH mechanism
go to slide
the rate of ATP production is higher in homolactic fermentation or oxidative phosphorylation
it is 100x faster in homolactic
detail alcoholic fermentation
pyruvate- pyruvate decarboxylase - acetaldehyde - alcohol dehydrogenase - ethanol
what is TPP and what is its deficiency?
it is synthesized from thiamine (VB3), it is a dietary requirement. The lack results in beriberi
what is the pyruvate decarboxylase mechanism?
go to slide
function of alcohol dehydrogenase is?
to reduce acetaldehyde and regenerate NAD+
what is the Warburg effect?
the metabolic rewiring of cancer cells that had a dysfunctional mitochondria and so even in the presence of oxygen used anaerobic glycolysis
