Final; Efficacy of Diagnostic Techniques for Periodontal Disease Flashcards Preview

AU14 Periodontology > Final; Efficacy of Diagnostic Techniques for Periodontal Disease > Flashcards

Flashcards in Final; Efficacy of Diagnostic Techniques for Periodontal Disease Deck (26):
1

Periodontal disease is currently diagnosed almost entirely on the basis of what

its clinical manifestations; gingival inflammation, periodontal probing, tooth mobility, furcation involvement, radiographs for bone loss

2

What are the two traditionally used diagnostic procedures for diagnosing periodontal disease

periodontal examination and radiographs

3

What three tests are not routinely used in clinical practice

microbiologic testing
assessment of the host response
genetic analysis

4

What are some limitations of probe penetration

lack of sensitivity and reproducibility
probing depth; inflammation, technique, subgingival calculus

5

What are some limitations of diagnosing CAL

poor reliability and reproducibility
limited practical value

6

What are some limitations of radiographic examinations

limited sensitivity in small bone change; can only be identified by eye after 30% to 50% of the bone mineral has been lost
no value in evaluating disease activity or progression

7

What is the mechanism of ultrasonic periodontal probing

it uses a hollow tapered tip that is filled with water for coupling of the ultrasonic beam into the tissues (non-invasive)

8

This has been introduced for the detection of periodontal defects in in-vitro settings, especially for bony defects, periodontal cysts, furcation defects, and thickness of palatal masticatory mucosa

CBCT

9

This quality of a diagnostic test refers to the probability of the test being positive when the disease is truly present

sensitivity

10

This quality of a diagnostic test refers to the probability of the test being negative when the disease is not present

specificity

11

This type of microbiologic testing is the gold standard method which assess for antibiotic susceptibility of microbes but has low sensitivity

bacterial culturing

12

This type of microbiologic testing depicts the morphology and motility of bacteria in a plaque sample

direct microscopy

13

This type of microbiologic testing uses Abs that target sepcific bacterial Ag, flow cytomerty, ELISA, and latex agglutination

immunodiagnositc methods

14

This type of microbiologic testing identifies common tyrpsin-like enzymes in periodontal pathogens

enzymatic methods

15

This type of microbiologic testing analyzes DNA, RNA, and structure and function or proteins from target microorganisms

molecular biology techniques

16

What are five sources of a sample used to asses the host response

saliva
GCF (most commonly used)
blood serum
blood cells
urine

17

How would you collect GCF

paper strips are placed within the crevice for 30 seconds

18

What three things can be found within the GCF

host-derived enzymes and their inhibitors
byproducts of tissue breakdown
inflammatory mediators and host-response modifiers

19

These are possible markers of active periodontal destruction, released from dead or dying PMNs

intracellular destruction enzymes

20

What are four examples of intracellular destruction enzymes

aspartate amino-transferase
alkaline phosphatase
beta-glucuronidase
elastase

21

These are associated with the activity of matrix metalloproteinases, produced by inflammatory, epithelial, and connective tissue cells

extracellular destruction enzymes

22

The ECM of the periodontium is composed of what

collagen, proteoglycans, and non-collagen proteins

23

Elevated levels of what three things can be found in the GCF from sites with periodontitis

hydroxyproline (collagen breakdown)
glycosaminoglyans (from matrix degradation)
osteocalcin and type I collagen (from alveolar bone destruction)

24

Why test saliva for periodontal disease

its abundant, easy to collect, and store
high enriched content of disease biomarkers

25

What two tests can be done via saliva testing

type and concentration of specific periodontal pathogens
genetic susceptibility based upon over-expression of IL-1 a

26

Whats the main issue with salivary diagnostic testing

not being able to specifically predict periods of disease activity