- white, cream or tan colonies - flat

- black, green, pink, not white - fluffy, tall growths

final lab practical Flashcards by sophie selander

how to collect fungassay sample

clean site (lay alcohol soaked gauze over site, remove and let air dry)
use hemostat to remove larger debris from edge of lesion
trim off piece and make fine shavings with a scalpel

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how to culture fungassay sample

inoculation: flame loop, collect sample, transfer to agar (do not bury or close lid completely to allow airflow)

incubation: room temp, monitor everyday for growth

interpretation: 2-3 days agar color change is complete; dermatophytes induce fast color changing and colonies will be small

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how to make fungassasy slide from culture

1-2 drops pf stain on slide
flame wire loop
use loop to remove growth from culture
mix with stain
place cover slip
10X or 40X to look on edges of clumps

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microsporum canis

zoonotic
dermatophyte

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microsporum gypseum

zoonotic
dermatophyte

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trichophyton mentagrophytes

zoonotic
dermatophyte

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fusarium

contaminant

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alternaria

contaminant

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penicillium

contaminant

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rhizopus

contaminant

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aspergillus fumigatus

contaminant

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yeast

contaminant

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conidia

an asexual spore

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hyphae

filaments that compose the body of a fungus

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mycelium

a mat made up of intertwining, thread-like hyphae

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dermatomycosis

skin disease caused by fungus

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fungal media

sabouraud dextrose agar
phenol red added

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dermatophyte color

white, cream or tan colonies
flat

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contaminant color

black, green, pink, not white
fluffy, tall growths

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calculating maximum ml of blood taken with survival

wt in grams x 0.01 = max ml

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bleeding sites for birds

jugular (right is bigger)
ulnar
medial metatarsal
skin prick (least acceptable)

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injection sites for birds

PO (into crop)
IM (pectoral muscles)
IV (jugular, metatarsal)
IO (distal ulna)

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bleeding sites for rabbits

IC
marginal ear vein
central ear artery
lateral saphenous
jugular
cephalic

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injection sites for rabbits

IV (marginal ear, cephalic)
PO (placed in diastema)
SQ (avoid neck)
IM (caudal thigh, paralumbar)
IP (head tilted down, lower left)

bleeding sites for guinea pigs

saphenous femoral blind stick cranial vena cava IC

injection sites for guinea pigs

SQ IM (anterior leg muscles) IP (head down, lower left) IV (marginal ear, sublingual, dorsal penile) PO

identification methods for mice

ear punch ear clip tattoo

identification methods for rats

ear punch tattoo

identification methods for hamsters

ear punch ear tag tattoo

identification methods for gerbils

ear punch ear tag tattoo

identification methods for birds

leg band wing band wing clip tattoo

identification methods for reptiles and amphibians

PIT tag beads

identification methods for guinea pigs

ear tag ear notch tattoo

identification methods for non-human primates

tattoo

identification methods for rabbits

ear clip tattoo in inner ear

identification methods for ferrets

PIT tag

housing (and behavior) for mice

crepuscular males individual, females in groups

housing (and behavior) for rats

nocturnal males together, females together

housing (and behavior) for hamsters

nocturnal - house individually - solid bottom (shoebox cage) - hibernate at low temps - calcium carbonate

housing (and behavior) for gerbils

diurnal - do not grab tail - little urination and defecation - less cleaning needed

housing (and behavior) for birds

twice length of wingspan or tail length small bathtub perches water cover nightly hide boxes

housing (and behavior) for R+A

POTZ UVA UVB hides water substrates

housing (and behavior) for guinea pigs

diurnal males separate, females together

housing (and behavior) for non-human primates

mechanical - squeeze cages for restraint

housing (and behavior) for rabbits

crepuscular ventilated on all sides extra cooling urine scale nest box, hay, straw litterbox trainable

housing (and behavior) for ferrets

narrow wire spacing solid bottom floor well ventilated prone to heat stroke and hairballs

TPR for rabbit

T: 101-104 F P: 300 bpm R: 32-60 rpm

TPR for bird

T:105 P: 150-200 R: 15-60

TPR for guinea pigs

T: 99-103 F P: 230-380 bpm R: 42-104 rpm

how to recognize a sick bird

unkept feathers, drooping posture/unsteadiness, decreased activity level, beak overgrowth, tail bobbing, not eating

how to recognize a sick rabbit

eyes/nose ears/skin behavior teeth GI

how to recognize a sick guinea pig

eyes nose ears skin behavior teeth GI

rabbit, bird, GP anatomy

quizlet

fungal ID

quizlet

hematology ID

quizlet

parasite stations

quizlet

sexing a rabbit and GP

quizlet

rabbit restraint

quizlet