Fiona Savoury Flashcards
(11 cards)
1
Q
Illumina sequences advantages
A
- High Throughput
- Low Error Rates
- Cost-Effectiveness
2
Q
Illumina sequencing disadvantages
A
- Short read length
- GC bias
- Higher input DNA requirements
3
Q
PacBio sequencing advantages
A
- Long Read Lengths
- Superior for Repetitive Regions
- High Sensitivity for Low-Coverage Applications
4
Q
PacBio sequencing disadvantages
A
- Higher Cost
- Lower Throughput
5
Q
Oxford NanoPore sequencing advantages
A
- Long Read Lengths
- Affordable
- High Throughput
6
Q
Genome coverage
A
- is this an average across the whole genome?
- there are key sites of the genome, where it is crucial to have high coverage, in CRISPR experiments
7
Q
further research
A
- functional gene characterisation
- gene phylogeny
- KO and KI
8
Q
if they haven’t done something, think about why?
A
- is it difficult? expensive? time-consuming?
9
Q
genome
A
- is it draft or reference
- to what degree is it functionally annotated?
9
Q
models sp.
A
how do they translate into crops?
10
Q
functional annotation
A
why did something evolve?
