First Aid General Bacteriology

Question 1

What media is used for isolation of lactose-fermenting bugs?

Answer 1

MacConkey agar

Fermentation produces acid, causing colonies to turn pink.

Question 2

Chocolate agar is used for isolation of what bug?

Answer 2

H. influenzae

Question 3

What media is used for isolation of Legionella?

Answer 3

Charcoal yeast extract agar buffered with cysteine and iron

Question 4

Tellurite agar and Löffler medium are used to isolate what bug?

Answer 4

C. diphtheriae

Question 5

Löwenstein-Jensen agar is used for isolation of what bug?

Answer 5

M. tuberculosis

Question 6

Eosin–methylene blue (EMB) agar is used for isolation of what bug?

Answer 6

E. coli

Colonies with green metallic sheen

Question 7

What are the media contents of Bordet-Gengou agar?

Answer 7

potato

(Bordet-Gengou is red, Regan-Lowe is black)

Question 8

Charcoal yeast extract agar buffered with cysteine and iron is used for isolation of what bug?

Answer 8

Legionella

Question 9

Bordet-Gengou agar (Bordet for Bordetella) and Regan-Lowe medium are used for isolation of what bug?

Answer 9

B. pertussis

(Bordet-Gengou is red, Regan-Lowe is black)

Question 10

What media is used for isolation of N. gonorrhoeae and N. meningitidis?

Answer 10

Thayer-Martin agar

Question 11

What media is used for isolation of B. pertussis?

Answer 11

• Bordet-Gengou agar (Bordet for Bordetella) (red)
• Regan-Lowe medium (black)

Question 12

What media is used for isolation of M. tuberculosis?

Answer 12

Löwenstein-Jensen agar

Question 13

Eaton agar is used for isolation of what bug?

Answer 13

M. pneumoniae

Question 14

What media is used for isolation of E. coli?

Answer 14

Eosin–methylene blue (EMB) agar

Colonies with green metallic sheen

Question 15

Thayer-Martin agar is used for isolation of what bugs?

Answer 15

N. gonorrhoeae, N. meningitidis

Question 16

What media is used for isolation of C. diphtheriae?

Answer 16

Tellurite agar, Löffler medium

Question 17

Sabouraud agar is used for isolation of what bugs?

Answer 17

Fungi

“Sab’s a fun guy!”

Question 18

What media is used for isolation of H. influenzae?

Answer 18

Chocolate agar

Question 19

What are the media contents of Regan-Lowe medium?

Answer 19

Charcoal, blood, and antibiotic

(Bordet-Gengou is red, Regan-Lowe is black)

Question 20

Factors V (NAD+ and X (hematin) are the media contents of what bacterial growth media?

Answer 20

chocolate agar

Question 21

What media is used for isolation of M. pneumoniae?

Answer 21

Eaton agar

Question 22

What are the media contents of Thayer-Martin agar?

Answer 22

• Vancomycin (inhibits gram-positive organisms),
• Trimethoprim,
• Colistin (inhibits gram-negative organisms except Neisseria), and
• Nystatin (inhibits fungi)

Very Typically Cultures Neisseria

Question 23

MacConkey agar is used for isolation of what bugs?

Answer 23

Lactose-fermenting enterics
Fermentation produces acid, causing colonies to turn pink.