Flashcards in From golgi to lysosome Deck (14):
1
What is the pH of lysosomes?
4.5-5
2
How is the low pH maintained?
H+ pump (vacuolar H+ pump in membrane)
3
how are digested material transported to the membrane?
H+ gradient (secondary transport)
specific transport proteins
4
What is used to degrade the material in lysosomes?
acidic hydrolases
5
What is the mannose 6-phosphate receptor used for?
it recognizes lysosomal hydrolases tagged with M6P in the TGN
6
What signals for the addition of M6P?
a signal sequence
7
What mediates the addition of of M6P?
N-acetylglucosaamine (GlcNAc) phosphotransferase
GlcNAc phosphoglycosidase
8
Where is the M6P receptor?
shuttles back and forth between membranes
9
What two types of receptors mediate M6P sorting to lysosomes?
CI-M6PR mediates cell surface IGF-II binding through short high affinity peptide sequences in IGF-II
it can also bind M6P moieties and deliver them to the lysosome
10
What is the pathway of hydrolases?
ER-> Golgi-> endosomes ->lysosomes
11
What is the pathway of endocytosis?
early endosomes -> late endosomes -> lysosomes
12
What is the pathway of phagosytosis?
Phagosomes ->lysosomes
13
What is the pathway of autophagy?
autopphagosomes -> lysosomes
14