Flashcards in From golgi to lysosome Deck (14):
What is the pH of lysosomes?
How is the low pH maintained?
H+ pump (vacuolar H+ pump in membrane)
how are digested material transported to the membrane?
H+ gradient (secondary transport)
specific transport proteins
What is used to degrade the material in lysosomes?
What is the mannose 6-phosphate receptor used for?
it recognizes lysosomal hydrolases tagged with M6P in the TGN
What signals for the addition of M6P?
a signal sequence
What mediates the addition of of M6P?
N-acetylglucosaamine (GlcNAc) phosphotransferase
Where is the M6P receptor?
shuttles back and forth between membranes
What two types of receptors mediate M6P sorting to lysosomes?
CI-M6PR mediates cell surface IGF-II binding through short high affinity peptide sequences in IGF-II
it can also bind M6P moieties and deliver them to the lysosome
What is the pathway of hydrolases?
ER-> Golgi-> endosomes ->lysosomes
What is the pathway of endocytosis?
early endosomes -> late endosomes -> lysosomes
What is the pathway of phagosytosis?
What is the pathway of autophagy?
autopphagosomes -> lysosomes