Fundamentals of Biochemical reactions Flashcards Preview

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Flashcards in Fundamentals of Biochemical reactions Deck (23):
1

What will inhibit metalloenzymes?

Chelating enzymes, take the metal away

2

What is the troponin subunit that is used to detect myocardial infarction? When is maximum sensitivy?

cTn-1: subunit only in cardiac muscle
10-24 hours following

3

What is the formula for the gastric proton pump? Where is it found?
What do the protons combine with when pumped out?
What do proton pump inhibitors essentially do?
What type of enzyme can these reduce the efficiency of?

H+ out/K+ in ATPase
Parietal cells in gastric lumen
HCl
Reduce HCl production
Gastric enzyme effiiency

4

What is an oxidoreductase? What does Lactate Dehydrogenase do?

Enzyme that does oxidation reduction.
Turn Pyruvate to Lactate

5

What is a addition elimination reaction?

Transfer of an atom to a multiple bond or elimination of an atom to form multiple bond

6

What does lead do to body? What are symptoms? How do you get rid of it?

Lead inhibits heme biosynthesis, rbs not formed you get anemia. Abdominal pain, anemia. Give ca-edta chelator, lead likes chelator more.

7

What do cofactors do for enzymes and what type of interaction?

Stabilize active site, noncovalent interaction.

8

What are the y intercept, x intercept, and slope for lineweaver burk?

Y intercept = 1/Vmax
X intercept = -1/Km
Slope = Km/Vmax

9

What are the two types of Coenzymes? What are coenzymes made from?

Co-substrates: Temporary association
Prosthetics: Permanent association
Made from Vitamins.

10

What are the two physiological buffers and equations?

Acetic acid CH3COOH
Bicarbonate HCO3- + H+ H2CO3 CO2 +H2O

11

Physiological PH?

7.37-7.43

12

Optimal body temp and ph for enzymes

Body temp: 37 C
pH: 4-8

13

Name a enzyme for each cofactor: Cu, Fe, Mg, Se, Zn

Cu: Cytochrome c Oxidase
Fe: Heme proteins
Mg: ATPases
Se: Glutathione Peroxidase
Zn: Superoxide dismutase

14

Match Respiratory Acidosis/Alkalosis with Hyper and Hypoventilation

Respiratory Acidosis=Hypoventilation
Respiratory Alkalosis= Hyperventilation

15

Isozymes can be used as markers of ___?
Isozymes have same function but different ____?

Myocardial infarction
Primary sequence

16

Irreversible enzyme inactivator effects on Km/Vmax

Vmax lowered, no change in Km

17

How does the Kidneys regulate the blood PH?

Remove H+ from blood forming NH4+, this reabsorbs HCO3-. This process is ph dependent.

18

Free energy equations

ΔG=ΔG* + RTlnQ
ΔG*= -RTlnKeq

19

For uncompetitive inhibitors, what happens to KM and Vmax

Vmax down, Km down

20

For Non competitive inhibitors, what happens to KM and Vmax

Vmax down, KM same

21

For enzyme, Ea is minimum amount of energy needed to convert ____ to _____.

Substrate to intermediate

22

For competitive inhibitors, what happens to KM and VMAX?

Vmax same, Km up

23

Equation for the Lineweaver Burk?

1/V = Km/(Vmax * S) + 1/Vmax