Flashcards in Fundamentals of Biochemical reactions Deck (23):
What will inhibit metalloenzymes?
Chelating enzymes, take the metal away
What is the troponin subunit that is used to detect myocardial infarction? When is maximum sensitivy?
cTn-1: subunit only in cardiac muscle
10-24 hours following
What is the formula for the gastric proton pump? Where is it found?
What do the protons combine with when pumped out?
What do proton pump inhibitors essentially do?
What type of enzyme can these reduce the efficiency of?
H+ out/K+ in ATPase
Parietal cells in gastric lumen
Reduce HCl production
Gastric enzyme effiiency
What is an oxidoreductase? What does Lactate Dehydrogenase do?
Enzyme that does oxidation reduction.
Turn Pyruvate to Lactate
What is a addition elimination reaction?
Transfer of an atom to a multiple bond or elimination of an atom to form multiple bond
What does lead do to body? What are symptoms? How do you get rid of it?
Lead inhibits heme biosynthesis, rbs not formed you get anemia. Abdominal pain, anemia. Give ca-edta chelator, lead likes chelator more.
What do cofactors do for enzymes and what type of interaction?
Stabilize active site, noncovalent interaction.
What are the y intercept, x intercept, and slope for lineweaver burk?
Y intercept = 1/Vmax
X intercept = -1/Km
Slope = Km/Vmax
What are the two types of Coenzymes? What are coenzymes made from?
Co-substrates: Temporary association
Prosthetics: Permanent association
Made from Vitamins.
What are the two physiological buffers and equations?
Acetic acid CH3COOH
Bicarbonate HCO3- + H+ H2CO3 CO2 +H2O
Optimal body temp and ph for enzymes
Body temp: 37 C
Name a enzyme for each cofactor: Cu, Fe, Mg, Se, Zn
Cu: Cytochrome c Oxidase
Fe: Heme proteins
Se: Glutathione Peroxidase
Zn: Superoxide dismutase
Match Respiratory Acidosis/Alkalosis with Hyper and Hypoventilation
Respiratory Alkalosis= Hyperventilation
Isozymes can be used as markers of ___?
Isozymes have same function but different ____?
Irreversible enzyme inactivator effects on Km/Vmax
Vmax lowered, no change in Km
How does the Kidneys regulate the blood PH?
Remove H+ from blood forming NH4+, this reabsorbs HCO3-. This process is ph dependent.
Free energy equations
ΔG=ΔG* + RTlnQ
For uncompetitive inhibitors, what happens to KM and Vmax
Vmax down, Km down
For Non competitive inhibitors, what happens to KM and Vmax
Vmax down, KM same
For enzyme, Ea is minimum amount of energy needed to convert ____ to _____.
Substrate to intermediate
For competitive inhibitors, what happens to KM and VMAX?
Vmax same, Km up