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Bacteriology > General media (LAB) > Flashcards

General media (LAB) Flashcards

1
Q

MacConkey agar lactose fermenting colonies color

A

Pink

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2
Q

MTM

A

Modified Thayer- Thayer Martin

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3
Q

provides an indication of the type of organism
present based on oxygen requirements

A

SEMI-SOLID

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4
Q

MTM is selective for!l?

A

N. gonorrohoeae and N. meningiditis

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5
Q

Composed of soy broth

A

Thioglycollate broth

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6
Q

composed of mannitol as a primary carbon source

A

GN broth

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7
Q

pH indicator for MacConkey Agar

A

neutral red

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8
Q

GN

A

Gram Negative Broth

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9
Q

pH indicators for Hektoen enteric agar

A

bromthymol blue & acid fuchsin

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10
Q

Agarose need to be cooled between this temperature for distribution into petri dishes

A

55-60 deg. celsius

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11
Q

Non lactose fermenter color with HE agar

A

Green

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12
Q

Inhibits GN in XLD agar

A

Sodium deoxycholate

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13
Q

most common solidifying agent

A

Agarose

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14
Q

Cultivation of non-fastidious microorganism, determination of hemolytic reactions

A

Sheep Blood Agar

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15
Q

PEA

A

Phenylethyl Alcohol

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16
Q

Enrichment broth or semi-solid, supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms

A

Thioglycollate Broth

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17
Q
A
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18
Q

bacterial growth is indicated by turbidity

A

Broth media

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19
Q

Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli with phenol red as the pH indicator

A

XLD

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20
Q

Blood agar based enriched with hemoglobin and supplement B

A

MTM

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21
Q

Components for BHI

A

Dextrose
Pork brain
Heart dehydrated infusion

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22
Q

MA

A

MacConkey Agar

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23
Q

Use to inhibit Proteus spp. in MTM agar

A

Trimethoprim

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24
Q

Agarose re-solidifies on this temperature

A

below 50 deg. celsius

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25
Q

composed pancreatic digest of casein

A

Thioglycollate broth

26
Q

Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.

A

MTM

27
Q

Cultivation of non-fastidious
microorganism, determination of hemolytic
reactions

A

SHEEP BLOOD AGAR (BA)

28
Q

BHI

A

Brain Heart Infusions

29
Q
  • needs are relatively complex, and exceptional
    media components must be used for growth
A

fastidious

30
Q

Peptones sources , 5%
defibrinated sheep blood

A

COLUMBIA CNA WITH BLOOD

31
Q

Isolation and differentiation of lactose
fermenting and non–lactose- fermenting enteric bacilli

A

MACCONKEY AGAR

32
Q

Selective isolation of gram-positive
cocci

A

COLUMBIA CNA WITH BLOOD

33
Q

BA

A

Sheep Blood Agar

34
Q

Selective isolation of aerobic gram-positive
cocci and bacilli

A

Phenylethyl Alcohol Agar

35
Q

Components of Thioglycollate broth

A

Primary digest of casein
Glucose
Soy broth

36
Q

MacConkey Agar non lactose fermenting colonies color

A

Colorless

37
Q
  • contain inhibitory agents against a specific bacteria,
    while allowing only a “selected” bacteria to grow
A

SELECTIVE MEDIA

38
Q

XLD

A

Xylose-Lysine-Deoxycholate

39
Q

BA: Cultivation of non-fastidious microorganism, determination of ______

A

hemolytic reactions

40
Q

a component of Columbia CNA w/ blood that blocks DNA replication

A

nalidixic acid

41
Q

inhibits GN in PEA agar

A

phenylethyl alcohol

42
Q

HE

A

Hektoen Enteric Agar

43
Q

Vancomycin in MTM is use to?

A

Inhibit GP

44
Q

Artificial environment where needs
of bacterial organisms are met to
proliferate and grow

A

MEDIA

45
Q

Selective for N.gonorrohoeae and
N.meningitidis. Supports the growth of Francisella and
Brucella spp.

A

Modified Thayer-Thayer Martin Agar

46
Q

pH indicator of XLD

A

Phenol red

47
Q

CA

A

Chocolate Agar

48
Q

h2s indicator

A

ferric ammonium citrate

49
Q

supports growth of most non-fastidious organism

A

NUTRITIVE MEDIA

50
Q
  • used to enhance growth of a particular bacteria by
    providing specific nutrients required
A

ENRICHMENT MEDIA

51
Q

Agarose melts in this temperature

A

95 deg Celsius

52
Q

Thioglycollate broth is use as an _____ broth or semi-solid, supports growth of ___,______,_____, and fastidious microorganisms

A

Enrichment
Anaerobes, aerobes, microaerophilic

53
Q

Cultivation of fastidious microorganisms
such as Haemophilus spp., Brucella spp. and
pathogenic Neisseria spp

A

CHOCOLATE AGAR

54
Q

A broth agar for cultivation of fastidious organisms

A

Brain Heart Infusion

55
Q

H2s indicator in XLD

A

Ferric ammonium citrate

56
Q

Nystatin in MTM is use to?

A

Inhibit yeast

57
Q

Collistin in MTM is use to?

A

Inhibit GN

58
Q
  • disrupts GN cell membrane in Colombia CNA w/ blood agar
A

Colistin

59
Q

Peptone base, enriched solution of
hemoglobin

A

CHOCOLATE AGAR

60
Q

Made of Trypticase soy agar

A

BA

61
Q

Selective (enrichment) liquid
medium for gastrointestinal pathogens

A

GRAM NEGATIVE (GN) BROTH

62
Q

used to observe and detect the motility of
bacteria

A

SEMI-SOLID

Bacteriology (11 decks)

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