General media (LAB)

Question 1

MacConkey agar lactose fermenting colonies color

Answer 1

Pink

Question 2

MTM

Answer 2

Modified Thayer- Thayer Martin

Question 3

provides an indication of the type of organism
present based on oxygen requirements

Answer 3

SEMI-SOLID

Question 4

MTM is selective for!l?

Answer 4

N. gonorrohoeae and N. meningiditis

Question 5

Composed of soy broth

Answer 5

Thioglycollate broth

Question 6

composed of mannitol as a primary carbon source

Answer 6

GN broth

Question 7

pH indicator for MacConkey Agar

Answer 7

neutral red

Question 8

GN

Answer 8

Gram Negative Broth

Question 9

pH indicators for Hektoen enteric agar

Answer 9

bromthymol blue & acid fuchsin

Question 10

Agarose need to be cooled between this temperature for distribution into petri dishes

Answer 10

55-60 deg. celsius

Question 11

Non lactose fermenter color with HE agar

Answer 11

Green

Question 12

Inhibits GN in XLD agar

Answer 12

Sodium deoxycholate

Question 13

most common solidifying agent

Answer 13

Agarose

Question 14

Cultivation of non-fastidious microorganism, determination of hemolytic reactions

Answer 14

Sheep Blood Agar

Question 15

PEA

Answer 15

Phenylethyl Alcohol

Question 16

Enrichment broth or semi-solid, supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms

Answer 16

Thioglycollate Broth

Question 18

bacterial growth is indicated by turbidity

Answer 18

Broth media

Question 19

Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli with phenol red as the pH indicator

Answer 19

XLD

Question 20

Blood agar based enriched with hemoglobin and supplement B

Answer 20

MTM

Question 21

Components for BHI

Answer 21

Dextrose
Pork brain
Heart dehydrated infusion

Question 22

MA

Answer 22

MacConkey Agar

Question 23

Use to inhibit Proteus spp. in MTM agar

Answer 23

Trimethoprim

Question 24

Agarose re-solidifies on this temperature

Answer 24

below 50 deg. celsius

Question 25

composed pancreatic digest of casein

Answer 25

Thioglycollate broth

Question 26

Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.

Answer 26

MTM

Question 27

Cultivation of non-fastidious microorganism, determination of hemolytic reactions

Answer 27

SHEEP BLOOD AGAR (BA)

Question 28

BHI

Answer 28

Brain Heart Infusions

Question 29

- needs are relatively complex, and exceptional media components must be used for growth

Answer 29

fastidious

Question 30

Peptones sources , 5% defibrinated sheep blood

Answer 30

COLUMBIA CNA WITH BLOOD

Question 31

Isolation and differentiation of lactose fermenting and non–lactose- fermenting enteric bacilli

Answer 31

MACCONKEY AGAR

Question 32

Selective isolation of gram-positive cocci

Answer 32

COLUMBIA CNA WITH BLOOD

Question 33

BA

Answer 33

Sheep Blood Agar

Question 34

Selective isolation of aerobic gram-positive cocci and bacilli

Answer 34

Phenylethyl Alcohol Agar

Question 35

Components of Thioglycollate broth

Answer 35

Primary digest of casein Glucose Soy broth

Question 36

MacConkey Agar non lactose fermenting colonies color

Answer 36

Colorless

Question 37

- contain inhibitory agents against a specific bacteria, while allowing only a “selected” bacteria to grow

Answer 37

SELECTIVE MEDIA

Question 38

XLD

Answer 38

Xylose-Lysine-Deoxycholate

Question 39

BA: Cultivation of non-fastidious microorganism, determination of ______

Answer 39

hemolytic reactions

Question 40

a component of Columbia CNA w/ blood that blocks DNA replication

Answer 40

nalidixic acid

Question 41

inhibits GN in PEA agar

Answer 41

phenylethyl alcohol

Question 42

HE

Answer 42

Hektoen Enteric Agar

Question 43

Vancomycin in MTM is use to?

Answer 43

Inhibit GP

Question 44

Artificial environment where needs of bacterial organisms are met to proliferate and grow

Answer 44

MEDIA

Question 45

Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.

Answer 45

Modified Thayer-Thayer Martin Agar

Question 46

pH indicator of XLD

Answer 46

Phenol red

Question 47

CA

Answer 47

Chocolate Agar

Question 48

h2s indicator

Answer 48

ferric ammonium citrate

Question 49

supports growth of most non-fastidious organism

Answer 49

NUTRITIVE MEDIA

Question 50

- used to enhance growth of a particular bacteria by providing specific nutrients required

Answer 50

ENRICHMENT MEDIA

Question 51

Agarose melts in this temperature

Answer 51

95 deg Celsius

Question 52

Thioglycollate broth is use as an _____ broth or semi-solid, supports growth of ___,______,_____, and fastidious microorganisms

Answer 52

Enrichment Anaerobes, aerobes, microaerophilic

Question 53

Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp

Answer 53

CHOCOLATE AGAR

Question 54

A broth agar for cultivation of fastidious organisms

Answer 54

Brain Heart Infusion

Question 55

H2s indicator in XLD

Answer 55

Ferric ammonium citrate

Question 56

Nystatin in MTM is use to?

Answer 56

Inhibit yeast

Question 57

Collistin in MTM is use to?

Answer 57

Inhibit GN

Question 58

- disrupts GN cell membrane in Colombia CNA w/ blood agar

Answer 58

Colistin

Question 59

Peptone base, enriched solution of hemoglobin

Answer 59

CHOCOLATE AGAR

Question 60

Made of Trypticase soy agar

Answer 60

BA

Question 61

Selective (enrichment) liquid medium for gastrointestinal pathogens

Answer 61

GRAM NEGATIVE (GN) BROTH

Question 62

used to observe and detect the motility of bacteria

Answer 62

SEMI-SOLID