Flashcards in Glycolysis Deck (16):
Where does glycolysis take place in the cell?
What is a molecule with a six membered ring generally known as?
What is a molecule with a five membered ring generally known as?
What happens in a transamination reaction?
An amino group is transferred from one molecule to another (commonly from an amino acid to a keto acid)
What is broken down during phosphorylation in glycolysis?
ATP into ADP & Pi (inorganic phosphate molecule)
Why does the phosphorylation of glucose need to occur initially?
Phosphorylated form of glucose is ionised and therefore is trapped in the cell
What is NAD made up of?
Nicotinamide, ribose x2, adenine (ribose + adenine = adenosine)
Why is vitamin B3 so crucial in glycolysis?
It is extremely important in the manufacture of nicotinamide
Under aerobic conditions, what happens to the NADH produced?
It is sent to the electron transfer chain where it loses its H+, setting up a proton gradient which drives ATP synthase to manufacture ATP; when the hydrogen ion returns, it reacts with the final elector acceptor, oxygen, to produce water, which is then expelled from the cell. The remaining NAD- is recycled back to the start of the glycolysis reaction
Under anaerobic conditions, what happens to the NADH produced?
Due to the absence of a final electron acceptor (oxygen), the NADH is broken down to NAD in the reaction turning pyruvate into lactate, and recycled so that the process of glycolysis can continue, despite the anaerobic conditions
What part does hexokinase play in the rate regulation of glycolysis?
Glucose ---> Glucose-6-Phosphate
What part does phosphofructokinase play in the rate regulation of glycolysis?
Fructose-6-Phosphate ---> Fructose-1,6-Bisphosphate
What part does pyruvate kinase play in the rate regulation of glycolysis?
Phosphoenolpyruvate ---> Pyruvate
What is meant by the term 'allosteric inhibition'?
Inhibition of an enzyme by binding to a site distant to its active site, but causing a conformational change in its structure so that it can't bind to a substrate
Name three factors that inhibit the phosphofructokinase enzyme
High concentration of citric acid
High concentration of ATP