GPHT LAB - Apparatus I Flashcards Preview

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Flashcards in GPHT LAB - Apparatus I Deck (48):
1

machine on which thin sections are cut

microtome

2

process of using a microtome

microtomy

3

principle of microtomy

pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness

4

3 essential parts of a microtome

1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl

5

An essential part of the microtome used for actual cutting of tissue sections

knife and knife carrier

6

An essential part of the microtome where the tissue is held in position

block holder or chuck

7

An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections

ratchet feed wheel, adjustment screws, pawl

8

An essential part of the microtome adjusted or manipulated during microtomy

ratchet feed wheel, adjustment screws, pawl

9

types of microtome (5)

rotary (minot)
rocking
freezing
ultra-thin
sliding or base sledge

10

microtome invented by Caldwell Trefall

Rocking

11

microtome invented by Queckett

Freezing

12

microtome invented by Minot

Rotary (Minot)

13

microtome invented in 1881

Rocking

14

microtome invented in 1885 - 1886

Rotary (Minot)

15

microtome invented in 1848

freezing

16

microtome developed by Adams

Sliding or Base Sledge

17

microtome developed in 1789

Sliding or Base Sledge

18

microtome also known as Cambridge

Rocking

19

Rocking Microtome also known as

Cambridge

20

simplest of all microtomes

Rocking

21

what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block

Rocking

22

disadvantage of rocking microtome

will not produce GOOD serial sections

23

ribbons of how many sections are produced w/ a rocking microtome

60-90

24

thickness of the sections produced by a rocking micrometer in um

10-12 um

25

what type of microtome
relatively small length of knife is available for use; dangerously placed w/ the blade up

rotary (minot)

26

what type of microtome
not suitable for cutting large blocks

rotary (minot)

27

what type of microtome
for cutting serial sections

rotary (minot)

28

what type of microtome
most commonly used; present in the lab

rotary (minot)

29

what type of microtome
able to section paraffin-embedded sections

rotary (minot)

30

what type of microtome
consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder

freezing

31

in the freezing microtome, what allows the release of rapid intermittent burst of carbon dioxide

simple lever-operated valve

32

in the freezing microtome, what is the propellant or freezing agent

carbon dioxide

33

what type of microtome
used for rush frozen sections

freezing

34

what type of microtome
capable of cutting sections at 0.5 micron required for EM

ultra-thin

35

what type of microtome
knife contains selected fragments of broken plate glass

ultra-thin

36

what knife can be used for ultra-thin microtome

special diamond knife

37

what type of microtome
for cutting extremely hard blocks and for frozen sections

sliding or base sledge

38

what type of microtome
first microtome invented

sliding or base sledge

39

microtome knife is made of what?

stainless steel

40

histology technician's too of his profession

microtome knife

41

personal possession of histo tech

microtome knife

42

every technician should be given at least __ good knives

2

43

3 basic shapes of microtome knife

biconcave
plane concave
plane wedge

44

length of biconcave

120 mm

45

microtome knife used for paraffin sections with rocking or rotary type microtome

biconcave

46

microtome knife that can be used on a carbon dioxide freezing microtome

biconcave

47

microtome knife very useful for cutting paraffin tissue blocks

biconcave

48

biconcave knife is found on what microtome

rotary

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