GPHT LAB - Apparatus II Flashcards Preview

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Flashcards in GPHT LAB - Apparatus II Deck (42):
1

Shape of microtome knife
two sides: one flat and one concave

plane-concave

2

in plane concave knife, the one with lesser degree of concavity is used for __

celloidin section

3

in plane concave knife, the one with more degree of concavity is used for __

paraffin sections on sledge, rotary or rocking microtome

4

Shape of microtome knife
routinely used for frozen sections and extremely hard specimens embedded in paraffin wax

plane wedge

5

Shape of microtome knife
used for paraffin blocks being cut on the rotary microtome

plane wedge

6

length of plane wedge

100 mm

7

when not in use, where must the knife be stored

in its box

8

never lay the knife ___ on the bench

flat

9

never allow the knife to become badly " ___ "

nicked

10

it is advisable to have a 2nd knife for ___ and ___

cutting bone and hard tissue

11

the edge of the knife should be "___" daily before use

touch up

12

always use a ___, when required for sharpening

back

13

using a back for sharpening not necessary for what knives?

biconcave

14

cleaning material for the knife

xylene or toluene

15

never __ or __ a knife

lend or borrow

16

knife sharpener with a vibrating frosted glass plate

automatic knife sharpener

17

in the automatic knife sharpener, every __ strokes the blade is automatically turned over

4

18

automatic knife sharpener require the use of ___

abrasives

19

also called Cold Microtome

Cryostat

20

a cold chamber, which is kept at -20C

Cryostat

21

cold chamber of cryostat contains what microtome type

rotary

22

average temperature of the cold chamber/cryostat

-20C

23

used to cut frozen tissue

cryostat

24

replaced the carbon dioxide microtome for frozen sections

cryostat

25

thickness of tissue that can be cut by a cryostat

1-4 u

26

apparatus to prepare frozen tissue

cryostat

27

apparatus used to determine if tumor is benign or maignant

cryostat

28

RFS takes how many min

15 - 20

29

reagent in stations 1&2 of autotechnicon

10% formalin

30

an automatic tissue processor that performs fixation, dehydration clearing and paraffin infiltration

autotechnicon

31

adv of autotechnicon

constant agitation

32

steps performed by autotechnicon (4)

fixation, dehydration, clearing and paraffin infiltration

33

autotechnicon has how many contianers

12

34

reagent in stations 3-6of autotechnicon

ascending grades of ethyl alcohol (70% to 95%)

35

reagent in stations 7-8 of autotechnicon

2 changes of acetone

36

reagent in stations 9-10 of autotechnicon

2 changes of chloroform or xylol

37

reagent in stations 11&12 of autotechnicon

2 changes of liquid paraffin

38

parts of autotechnicon
for the specimen

sample basket

39

parts of autotechnicon
contains reagents

receptacle

40

parts of autotechnicon
enables the operator to delay the beginning of the processing cycle for any desired time up to 60hours

delay timer

41

parts of autotechnicon
contains power switch, selector, pilot light, transparent plastic door with lock and key

timing disc panel

42

parts of autotechnicon
temp at +/- 0.7C controlled by a thermostat

melting pots

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