GPHT LAB - Clearing Flashcards Preview

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Flashcards in GPHT LAB - Clearing Deck (71):
1

in dealcoholization, tissue section is immersed in __

ante-medium

2

raises the refractive index of the tissue

ante-medium

3

clearing agents must be miscible with both __

alcohol and paraffin wax

4

does not cause to much brittleness

benzene

5

best routine clearing agent

benzene

6

can cause aplastic anemia

benzene

7

used for large, thick, hard tissues

chloroform

8

slow acting
toxic to liver
does not make tissues translucent
tissues tend to float

chloroform

9

used for cleaning knives

xylene

10

diluent in mounting medium

xylene

11

most common clearing agent

xylene

12

for tissues less than 5 mm

xylene

13

useful for paraffin embedding

xylene

14

becomes milky/turbid if water is still present (incomplete dehydration)

xylene

15

overclearing causes tissue to become

hard and brittle

16

sub for xylene

toluene

17

subs for xylene/benzene

toluene

18

for CNS, skin, smooth muscle, cytological work

cedar oil/cedar wood oil

19

can leave tissue for a very long time w/o dehydration

cedar oil/cedar wood oil

20

penetrates well = best clearing agent

cedar oil/cedar wood oil

21

expensive and turbid

cedar oil/cedar wood oil

22

SLOW
Makes tissue brittle
can be faked

clove oil

23

prevents clove oil from becoming brittle

oil of bergamot

24

does not extract aniline dyes

oil of bergamot

25

can be used with celloidin prep

oil of bergamot

26

spanish hop oil or thyme oil or mix

oil of origanum

27

dissolves out aniline dyes

oil of origanum

28

does not dissolve celloidin

oil of origanum

29

other names of oil of wintergreen

methyl salicylate, artificial oil

30

for delicate tissues bc hardening effect of alcohol can be avoided

oil of wintergreen

31

clearing time is indefinite

cedar oil/cedar wood oil
oil of wintergreen

32

must be soaked out by __ when oil of wintergreen is used for celloidin sections

chloroform/xylene

33

poor dehydrant, split by small amts of water

oil of wintergreen

34

oxidizes easily on standing

oil of wintergreen

35

must be soaked out by xylene or chloroform when used for celloidin sections

oil of wintergreen

36

for smooth muscles (2)

carbon disulfide
phenol

37

very inflammable with foul odor

carbon disulfide

38

subs of chloroform

carbon tetrachloride

39

adv of carbon tetrachloride

cheaper

40

for friable tissues

carbol-xylene

41

made by adding anhydrous crystals of phenol to xylene until no more dissolves

carbol-xylene

42

for moist and humid climates

carbol-xylene

43

corrosive

carbol-xylene

44

for cellular organs, friable tissues, skin, athermatous arteries, hard collagenous specimens

tetrahydrofuran

45

how many baths for THF

3

46

3 baths required

THF

47

colorless agents

xylene
THF

48

BP of THF

65C

49

FP of THF

-108.5C

50

Density of THF

0.8894

51

Wide-range solvent
Soluble in most solvents

THF

52

THF Forms __ with water

azeotrope

53

forms azeotrope with water

THF

54

Constant bp mixture (64C) consisting of 9% THF and 6% water

azeotrope

55

melted paraffin wax mixes with __ in all proportions

THF

56

superior to ordinary dehydrating agent

THF

57

shortens total prcessing time

THF

58

fumes may cause conjunctival irritation after about 6 mos
with offensive odor

THF

59

recommended for the eyes

terpineol

60

excellent clearing agent

High Test Aviation Lead-Free Gasoline

61

for double embedding techniques

methyl salicylate and methyl benzoate

62

Suitable for large tissue specimens, tough tissues (e.g. Skin, fibroid, decalcified tissues), nervous tissues, lymph nodes, embryos

Chloroform

63

Does not extract aniline dyes
Can be used with celloidin

Oil of Bergamot

64

Dissolves aniline
Does not dissolve celloidin

Oil of Origanum

65

Possible Components of Oil of Origanum (3)

Spanish Hop Oil
Thyme Oil
Turpentine

66

How to make carbol-xylene

Add anhydrous crystals of phenol to xylene until no more dissolves

67

Composition of azeotrope

9% THF
6% water

68

THF may cause conjunctival irritation after how many months

6

69

Clears from 95% alcohol

Terpineol

70

Good for paraffin and celloidin work
But volatile

Phenol

71

cause the least hardening of tissues (3)

cedar oil, gasoline, petroleum ether

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