GPHT LAB - Dehydration Flashcards Preview

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Flashcards in GPHT LAB - Dehydration Deck (42):
1

hastens dehydration process

heat

2

reagent that ensures complete dehydration

anhydrous copper sulfate

3

reagent which absorbs any water present

anhydrous copper sulfate

4

color change for anhydrous copper sulfate

colorless to whitish blue

5

commonly used solutions for dehydration

alcohol, acetone, dioxane

6

for delicate tissues, at what concentration must dehydration start

30%

7

also called grain alcohol

ethyl alcohol

8

also called wood alcohol

methyl alcohol

9

also called rubbing alcohol

isopropyl alcohol

10

alcohol: not expensive, not toxic

ethyl alcohol

11

what smears, use of MetOH

bone marrow and blood smears

12

disadv of MetOH

toxic, can cause blindness

13

disadv of Butyl alcohol

slow acting and not routinely used in lab

14

what smears, use of isopropyl alcohol

compression smears

15

use of Butyl alcohol

plant and animal microtechniques

16

used for most urgent biopsies

acetone method

17

rapid dehydrant but causes excessive shrinkage

acetone

18

length of dehydration when using acetone

1/2 to 2 hours

19

unique reagent

dioxane/ diethylene dioxide

20

dual purpose of dioxane/ diethylene dioxide

dehydrating and clearing agent

21

miscible with both water and molten paraffin wax

dioxane

22

Disadv of __
highly toxic
expensive
sections prone to fall out of surrounding wax

dioxane

23

should be used only in a well-ventilated lab
any residue should be washed down the sink

dioxane

24

excellent dehydrating and clearing agent

dioxane

25

2 Methods for dioxane

Weiseberger's
Graupner's

26

also a dehydrating and clearing agent aside from dioxane

tetrahydrofuran

27

can dissolve many substances including fats

tetrahydrofuran

28

miscible with lower alcohols, ether, chloroform, acetone, benzene, xylene

THF

29

less shrinkage and easier cutting of sections with fewer artifacts

THF

30

does not dissolve out aniline dyes

THF

31

most staining procedures give improved results with ___

THF

32

used as a dehydrating agent because of its rapid action without producing overhardening and distortion of tissues

cellusolve

33

combustible at 110F

cellusolve

34

Cellusolve is combustible at ___

110F

35

Disadv of cellusolve

highly toxic
can affect reproductive organs

36

used as a dehydrating agent as it removes water very rapidly and produces very little distortion and hardening of tissue

tri-ethyl phosphate

37

soluble in alcohol, ether, benzene, chloroform, acetone, xylene

tri-ethyl phosphate

38

Used both as dehydration and clearing agent

Dioxane
Tetrahydrofuran

39

Soluble in many substances like alcohol, ether, benzene, etc

Tetrahydrofuran
Tri-ethyl phosphate

40

Has fewer artifacts when used

Tetrahydrofuran

41

Another name for cellusolve

Ethylene glycol monoethylether

42

produces minimum tissue shrinkage

tri-ethyl phosphate

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