GPHT LAB - Histopathologic Techniques Flashcards Preview

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Flashcards in GPHT LAB - Histopathologic Techniques Deck (36):
1

process of indicating the number of specimen by means of a pencil, in order to properly identify the specimen
properly speaking is the first step in all histopathologic techniques

numbering

2

is the first step in all histopathologic techniques

properly speaking

3

process of preserving cells and tissue constituents in a condition identical to that existing during life and to do this is a way that will allow preparation of thin stained sections

fixation

4

process of water removal from the tissue prior to replacement by wax. water is immiscible with paraffin or wax

dehydration

5

process whereby the alcohol in the tissues is replaced by afluid that will dissolve the wax with which the tissue must be impregnated

clearing or dealcoholization

6

process that involves the impregnation of tissue with a medium that will fill the natural cavities, spaces, and interstices of the tissues

wax or paraffin impregnation

7

process that involves the use of molds for the purpose of setting the embedding medium to a sufficient consistency in order to allow the cutting of suitably thin sections w/o undue distortion, w/o alteration of spatial relationships of the tissue and cellular elements

embedding

8

facilitates sectioning

embedding

9

process that involves separation of one tissue block from another using sharp knife (performed only when one uses a compound embedding unit)

blocking

10

process that involves the cutting of excess wax (in thin slices to prevent the block from cracking) from the tissue block so that the block forms a four-sided prism or truncated pyramid opposite sides being parallel (this is most important if serial sections are desired)

trimming

11

process of cutting very thin slices of tissues accomplished with a microtome

sectioning

12

apparatus for sectioning

microtome

13

process w/c involves the use of dyes or stains for optically differentiating the cellular and tissue constituents and to determine the free chemical nature of some details in the cells (histochemical staining)

staining

14

process w/c involves the use of a medium and cover slip to facilitate the ease of handling and storage of slide and to prevent damage to the section

mounting

15

process of indicating the year and the specimen number on one end of the prepared slide for proper identification

labeling

16

used to indicate the number of the specimen

pencil

17

first step in all histopathologic techniques

properly speaking

18

allows for the preparation of thin stained sections

fixation

19

why is dehydration performed

water is immiscible with paraffin or wax
therefore water must be removed before impregnating the tissue with wax

20

purpose of embedding (2)

allow the cutting of thin sections without undue distortion and without alteration of spatial relationships of tissue and cellular elements
facilitates sectioning

21

instrument used in blocking

sharp knife

22

performed only when one uses a compound embedding unit

blocking

23

purpose of staining (2)

to optically differentiate the cellular and tissue constituents
to determine the free chemical nature of some details in the cells (histochemical staining)

24

purposes of mounting (2)

facilitate the ease of handling and storage of slide
to prevent damage to the section

25

purpose of labeling

proper identification

26

materials used in mounting

medium and cover slip

27

another name for dealcoholization

clearing

28

in dealcoholization, alcohol is replaced by what?

a fluid that will dissolve the wax with which the tissue must be impregnated

29

why use molds in embedding?

to set the embedding medium to a sufficient consistency

30

why set the medium to a sufficient consistency

to allow the cutting of suitably thin sections w/o undue distortion and w/o alteration of the spatial relationships of tissue and cellular elements

31

why must slices be thin when trimming

to prevent the block from cracking

32

shape of block in trimming (2)

four-sided prism or truncated pyramid opposite sides being parallel

33

this is most important if serial sections are desired

opposite sides of the block are parallel

34

determining the free chemical nature of some details in cells

histochemical staining

35

what must be indicated when labeling

specimen number and year

36

blocking is only performed when one uses a

compound embedding unit

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