GPHT LAB - Infiltration Flashcards Preview

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Flashcards in GPHT LAB - Infiltration Deck (80):
1

why impregnate

to give tx a firm consistency

2

best and most common infiltrating medium

paraffin wax

3

MP of pure PW

56C

4

__ not be overheated

PW

5

PW requires __ hrs of infiltration

.

6

PW must be __ prior to use

filtered

7

coarse filter for PW

Green's # 904

8

PW requires what equipment

paraffin oven

9

if PW is reused, heat at __ to remove water

100C

10

PW can be used how many times

2x

11

disadv of PW

not for fatty tissues

12

PW Substitute
paraffin + polymer

paraplast

13

MP of paraplast

56-57C

14

Quite similar to paraplast but MP is 56-58C

Embeddol

15

with a rubber what PW Substitute

tissue mat

16

harder than ordinary PW but MP = 46-48C

Esterwax

17

PW Substitute
Soluble in 95% EtOH

Esterwax

18

MP of Esterwax

46-48C

19

Ex. of water-soluble waxes

carbowax

20

Adv of carbowax

no need for dehydration and clearing

21

disadv of carbowax

expensive, tissue sections dissolved during flotation

22

microtome for carbowax

base sledge or sliding

23

Methods of Paraffin impregnation
Changes of PW, transfer tissue from one container to another

manual processing

24

Methods of Paraffin impregnation
performs steps 2-5

automatic tissue processing

25

Methods of Paraffin impregnation
fastest, performed at negative atmospheric pressure

vacuum method

26

celloidin also called

colloidion

27

wax for hollow large tx tend to collapse

celloidin

28

no heating required

celloidin

29

time for processing when using celloidin

days to weeks

30

2 methods for celloidin
for brain and bone tx

wet celloidin method

31

2 methods for celloidin
eyes

drycelloidin method

32

for enzyme studies, heat sensitive substances

gelatin

33

no dehydration and clearing required (2)

gelatin
carbowax

34

preservative of gelatin (to prevent mold growth)

1% phenol

35

hard medium: can cut very thick sections

plastic/resins

36

for preparing ultra-thin and EM sections

plastic/resins

37

widest application, for light and EM

Epoxy resins

38

why impregnate

to give tx a firm consistency

39

best and most common infiltrating medium

paraffin wax

40

MP of pure PW

56C

41

__ not be overheated

PW

42

PW requires __ hrs of infiltration

Less than 24 hours

43

PW must be __ prior to use

filtered

44

coarse filter for PW

Green's # 904

45

PW requires what equipment

paraffin oven

46

if PW is reused, heat at __ to remove water

100C

47

PW can be used how many times

2x

48

disadv of PW

not for fatty tissues

49

PW Substitute
paraffin + polymer

paraplast

50

MP of paraplast

56-57C

51

Quite similar to paraplast but MP is 56-58C

Embeddol

52

with a rubber what PW Substitute

tissue mat

53

harder than ordinary PW but MP = 46-48C

Esterwax

54

PW Substitute
Soluble in 95% EtOH

Esterwax

55

MP of Esterwax

46-48C

56

Ex. of water-soluble waxes

carbowax

57

Adv of carbowax

no need for dehydration and clearing

58

disadv of carbowax

expensive, tissue sections dissolved during flotation

59

microtome for carbowax

base sledge or sliding

60

Methods of Paraffin impregnation
Changes of PW, transfer tissue from one container to another

manual processing

61

Methods of Paraffin impregnation
performs steps 2-5

automatic tissue processing

62

Methods of Paraffin impregnation
fastest, performed at negative atmospheric pressure

vacuum method

63

celloidin also called

colloidion

64

wax for hollow large tx tend to collapse

celloidin

65

no heating required

celloidin

66

time for processing when using celloidin

days to weeks

67

2 methods for celloidin
for brain and bone tx

wet celloidin method

68

2 methods for celloidin
eyes

drycelloidin method

69

for enzyme studies, heat sensitive substances

gelatin

70

no dehydration and clearing required (2)

gelatin
carbowax

71

preservative of gelatin (to prevent mold growth)

1% phenol

72

hard medium: can cut very thick sections

plastic/resins

73

for preparing ultra-thin and EM sections

plastic/resins

74

widest application, for light and EM

Epoxy resins

75

Rarely used infiltrating medium

Gelatin

76

Why use preservative for gelatin

To prevent growth of molds

77

Does not require dehydration and clearing (2)

Carbowax
Gelatin

78

Components of plastic/resins

Acrylic
Polyester
Glycerol
Epoxy resins

79

Component of plastic/resin infiltrating medium with widest application, for LM and EM

Epoxy resin

80

filling up of empty spaces cavities using an impregnating medium

infiltration

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