Gram Positive Rods

Question 1

Listeria ID

Answer 1

catalase (+)
esculin (+)
beta hemolytic
tumbling motility at 25C, but NOT at 35C (umbrella)
cold enrichment

Question 2

Listeria infections

Answer 2

neonatal septicemia and sepsis

sepsis in IC host

Question 3

Corynebacterium ID

Answer 3

catalase (+)
beta hemolytic (+/-)
Chinese letters
Loefflers slants (metachromatic granules)
tellurite hydrolysis (tinsdale agar)
elek test determines toxin production

Question 4

Erysipelothrix ID

Answer 4

H2S (+)
TSI (+)
“test tube brush” growth in gelatin

Question 5

Erysipelothrix infection

Answer 5

infection in fisherman, butchers, and vets

Question 6

Bacillus ID

Answer 6

catalase (+)
esculin V
beta hemolytic (-/+)
ground glass texture

Question 7

Bacillus anthracis

Answer 7

obligate pathogen
non hemolytic
non motile
medusa head colonies (filamentous outgrowth)

Question 8

Bacillus cereus

Answer 8

opportunistic, food pathogen
beta hemolytic
motility (+)
large flat colonies (irregular edges)
lecithinase (+)
"fried rice"

Question 9

Corynebacterium diphtheriae

Answer 9

small pleomorphic rods with clubbed ends
black colonies on tinsdale
produces pseudomembrane on tonsils, uvula or soft palate

Question 10

Rhodococcus

Answer 10

pathogen associated with horses