Growth Media Reproduction Flashcards

1
Q

What are the four growth requirements for bacteria

A

Temperature
Gas o2
Oh 6.5-7.5 buffers
Nutriential

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2
Q

What dies the thermal death point do

A

Destroys all bacteria

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3
Q

Optimium

A

The temperature at which growth is best suited

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4
Q

Psychrophilic

A

Cold loving

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5
Q

Mesophilic

A

Medium temperature loving

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6
Q

Thermophilic

A

Heat loving

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7
Q

Halophilic

A

Salt loving bacteria

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8
Q

Aerobes

A

Oxygen loving bacteria (aerobic)

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9
Q

Anaerobes

A

Co2 loving anaerobic

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10
Q

Obligate

A

Strict or only

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11
Q

Facultative

A

Tolerating the gas

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12
Q

Obligate aerobe

A

Grow only in O2

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13
Q

Obligate anerobe

A

Grow only in co2

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14
Q

Facultative aerobe

A

Fundamentally an anaerobe but can grow in o2

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15
Q

Facultative anerobe

A

Can grow in O2

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16
Q

Four types of cultures

A

Agar plates
Agar slants
Broth-liquid
Stabs-gelatin

17
Q

Complex media

A

Media had extracts: year beef plants blood

18
Q

Reducing media

A

Has chemicals in it that combine to o2 to deplete oxygen in media

EXAMPLE !!!: thioglycollate

19
Q

Selective media

A

Chemicals to inhibit some types of bacteria while allowing others to grow

20
Q

Differential media

A

Media contains special sugars that differentiate bacteria used for identification

21
Q

Endo

A

Agar grows only gram negative if it contains lactose is has a pink color

22
Q

Mannitol salt agar

A

High salt
Pink
Hight sugar
Selective and differential

23
Q

Enrichment media

A

Additives are placed in media which would not be able to survive

24
Q

Organism grown in a candle jar

A

Neisseria

25
Q

Blood agar

A

All bacteria grow

26
Q

Nutrient agar

A

All bacteria grow

27
Q

Pea

A

Only gran positive grow

28
Q

Endo

A

Only gram negative grow

29
Q

Doubling timer

A

Time required for a cell to divide

30
Q

What are the four phases of growth

A

Lag
Log
Stationary
Death

31
Q

Lag phase

A

No reproduction zero growth

32
Q

Log phase

A

Active metabolism growth occurs

Sensitive to antibiotics

33
Q

Stationary phase

A

Growth=death

34
Q

Death

A

Organisms are dying rapidly