HEMA 2 LEC - Automation in haematology Flashcards Preview

► Med Misc 05 > HEMA 2 LEC - Automation in haematology > Flashcards

Flashcards in HEMA 2 LEC - Automation in haematology Deck (37):
1


Require some steps, as dilution of blood samples
Often measure only a small number of variables

Semi-automated instruments

2


Require only that an appropriate blood sample is presented to the instrument.
They can measure 8-20 variables including some new parameters which do not have any equivalent in manual methods.

Fully automated instruments

3

Sizing is based on

impedance technology.

4

Hb is measured automatically by a modification of the

manual (cyanide) method.

5

To reduce toxicity of Cyanide some systems replace it by a non-toxic material ?

Na- lauryl sulphate

6

The RBCs are counted automatically by two methods
(2)

Aperture impedance
light scattering technology

7

cells are counted as they pass in a stream through an aperture
What method of counting rbc

Aperture impedance:

8

Some problems which could be faced:
(3)

Two cells passing through the orifice at the same time, counted as one cell.
RBC agglutination(clump of cells)
Counting bubbles or other particles as cells.

9

allows either the PCV or the MCV to be determined.

Pulse height analysis

10

Fully automated multichannel instruments perform WBC counting by either
(3)

Impedance
Or light scattering
Or both.

11

Automated differential counters which are available now generally use _____ incorporated into a full blood counter rather than being standard alone differential counters

flow cytometry

12

3-part differential usually cont

Granulocytes or large cells
Lymphocytes or small cells
Monocytes(mononuclear cells) or (middle cells)

13

5-part classify cells to

Neutrophils
Eosinophils
Basophils
Lymphocytes
Monocytes

14

A sixth category designated “large unstained cells” include cells larger than normal and lack the peroxidase activity this include

Atypical lymphocytes
Various other abnormal cells.

15

Other counters identifies 7 categories including

Large immature cells(composed of blasts and immature granulocytes)
Atypical lymphocytes(including blast cells).

16

Analysis in diff count may be dependant on:


Volume of the cell
Other physical characteristics of the cells
Sometimes the activity of cellular enzymes such as peroxidase.

17

technologies used in diff count

Light scattering and absorbance
Impedance measurement

18

In general automated differential counters are favourable to the manual in 2 conditions

Exam of normal blood samples
Flagging of abnormal samples

19

An automated retic count can be performed using the fact that ____ Fluorescent cells can then be enumerated using a flowcytometer.

various fluoro-chromes combine with the RNA of the reticulocytes.

20

An automated retic counter also permits the assessment of since

retic maturity

21

the more immature reticulocytes have more RNA fluoresce more or less ?? strongly than the mature retics found normally in PB.

moe strongly

22

Automated instruments produce ______ which allow the presence of more than one population of cells to be appreciated

volume distribution histograms

23

Automated instruments produce volume distribution histograms which allow what?

the presence of more than one population of cells to be appreciated

24

variation in cell volume,

RDW

25

an equivalent of the microscopic assessment of the degree of anisocytosis

RDW

26

RDW is an equivalent of the microscopic assessment of the degree of ___

anisocytosis

27

The total WBC count is determined in whole blood in which __ have been lysed.

red cells

28

Automated differential counters which are available now generally use flow cytometry incorporated into a ____ rather than being standard alone differential counters

full blood counter

29

Monocytes other name (2)

mononuclear cells
middle cells

30

large cells

granulocytes

31

small cells

lymphocytes

32

6th category in diff count automated is called

“large unstained cells”

33

include cells larger than normal and lack the peroxidase activity

“large unstained cells”

34

Automated differential counters employing_____ classify far more cells than is possible with a manual differential count.

flow cytometry

35

Other parameters in PLT automated count include (3)

MPV
PDW
Plateletcrit= MPV x platelet count

36

formula for plateletcrit

MPV x PLT count

37

sysmex model

Sysmex XE-2100

Decks in ► Med Misc 05 Class (295):