HEMA 2 LEC - Cytochemistry

Question 1

In vitro staining of cells that allows microscopic examination of the cell’s chemical composition

Answer 1

Cytochemistry

Question 2

Differentiate cell lineage of blasts in acute leukemia

Answer 2

Cytochemistry

Question 3

are used if constituents are sensitive to heat, light, storage, and processing techniques

Answer 3

Fresh smears

Question 4

can be used for stable constituents

Answer 4

Aged specimens

Question 5

Types of Cytochemical Staining

Answer 5

Enzymatic

NonEnzymatic

Question 6

Stains alkaline phosphatase present in the neutrophil

Answer 6

Leukocyte Alkaline Phosphatase Stain

Question 7

Used to differentiate
Chronic myelogenous leukemia from leukemoid reaction
Polycythemia vera from secondary erythrocytosis

Answer 7

Leukocyte Alkaline Phosphatase Stain

Question 8

Normal range LAP

Answer 8

30-185

Question 9

Stains peroxidase in granules of neutrophils, eosinophils, and monocytes

Answer 9

Myeloperoxidase Stain

Question 10

Used to differentiate AML and AMML from ALL (2)

Answer 10

Myeloperoxidase Stain

Sudan Black B Stain

Question 11

Show similar results with Sudan Black B stain

Answer 11

Myeloperoxidase Stain WHEN Used to differentiate AML and AMML from ALL

Question 12

Peroxidase activity is ___ in Mature neutrophils in CML

Answer 12

inc

Question 13

Peroxidase activity is ___ in Infection, AML, myelodysplastic conditions

Answer 13

decreased

Question 14

Differentiate acute leukemias that are myeloid (M1, M2. M3) from monocytic origin (M5)

Answer 14

Esterases

Question 15

Stains mucoproteins, glycoproteins, and HMW carbohydrates in all cells except pronormoblast

Answer 15

Periodic Acid-Schiff

Question 16

Used for the diagnosis of ALL, some subtypes of AML, and DiGuglielmo’s disease

Answer 16

Periodic Acid-Schiff

Question 17

PAS Reactions

Granulocytes

Answer 17

Intensity of stain increases as cells mature

Question 18

PAS Reactions

Megakaryocytes

Answer 18

Finely diffuse stain

Question 19

PAS Reactions

Platelets

Answer 19

Intensely red pink

Question 20

PAS Reactions
M6
Early erythroblast –

Answer 20

course and granular

Question 21

PAS Reactions

M6 Late erythroblast –

Answer 21

diffusely positive

Question 22

Lipids like sterols, phospholipids, neutral fats in monocytes and granulocytes

Answer 22

Sudan Black B Stain

Question 23

WHAT STAIN

Hairy cell leukemia

Answer 23

Acid phosphatase stain (with tartrate resistance

Question 24

WHAT STAIN

Differentiate granulocytic from monocytic leukemia

Answer 24

Nonspecific esterase stain (with fluoride inhibition)

Question 25

WHAT STAIN | Esterases in granulocytic cells

Answer 25

Chloroacetate esterase stain

Question 26

WHAT STAIN | Screening procedure for metabolic defects in neutrophil

Answer 26

Nitroblue tetrazolium (NBT)

Question 27

Dec LAP Activity

Answer 27

``` CML - Chronic Myelogenous Leukemia Sickle Cell Anemia Sideroblastic Leukemia Paroxysmal Nocturnal Hemoglobinuria Marked eosinophilia Infectious Mononucleosis ```

Question 28

Inc LAP Activity (LIP PC MOHAM)

Answer 28

``` Leukemoid reaction Infection pregnancy (last trimester) polycythemia vera corticosteroid therapy myelosclerosis obstructive jaundice hodgkin's disease aplastic leukemia multiple myeloma ```

Question 29

Normal LAP activity (LUVS)

Answer 29

Untreated hemolytic anemia Lymphosarcoma Viral hepatitis Secondary erythrocytosis

Question 30

LAP Score | 0

Answer 30

No staining

Question 31

LAP Score | 1+

Answer 31

faint and diffuse staining

Question 32

LAP Score | 2+

Answer 32

pale with moderate blue staining

Question 33

LAP Score | 3+

Answer 33

strong blue precipitated staining

Question 34

LAP Score | 4+

Answer 34

deep blue or brilliant staining with no visible cytoplasm

Question 35

Myeloperoxidase Stain stains granules in which cells

Answer 35

neutrophils, eosinophils and monocytes

Question 36

appearance of basophil and lymphocyte with myeloperoxidase stain

Answer 36

unstained

Question 37

Periodic Acid-Schiff Stains _____ (3) in all cells except pronormoblast

Answer 37

mucoproteins, glycoproteins, and HMW carbohydrates

Question 38

PAS used in the dx of (3)

Answer 38

ALL, some subtypes of AML, and DiGuglielmo’s disease

Question 39

PAS Rxns of lymphocytes in all

Answer 39

vary staining pattern, may be coarse or fine diffuse

Question 40

PAS rxns of L3 or Burkitt

Answer 40

PAS negative

Question 41

PAS negative

Answer 41

L3 or Burkitt

Question 42

Sudan Black B stain stains Lipids like sterols, phospholipids, neutral fats in what cells (2)

Answer 42

monocytes and granulocytes