HEMA 2 LEC - Cytochemistry Flashcards Preview

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Flashcards in HEMA 2 LEC - Cytochemistry Deck (42):
1

In vitro staining of cells that allows microscopic examination of the cell’s chemical composition

Cytochemistry

2

Differentiate cell lineage of blasts in acute leukemia

Cytochemistry

3

are used if constituents are sensitive to heat, light, storage, and processing techniques

Fresh smears

4

can be used for stable constituents

Aged specimens

5

Types of Cytochemical Staining

Enzymatic
NonEnzymatic

6

Stains alkaline phosphatase present in the neutrophil

Leukocyte Alkaline Phosphatase Stain

7

Used to differentiate
Chronic myelogenous leukemia from leukemoid reaction
Polycythemia vera from secondary erythrocytosis

Leukocyte Alkaline Phosphatase Stain

8

Normal range LAP

30-185

9

Stains peroxidase in granules of neutrophils, eosinophils, and monocytes

Myeloperoxidase Stain

10

Used to differentiate AML and AMML from ALL (2)

Myeloperoxidase Stain
Sudan Black B Stain

11

Show similar results with Sudan Black B stain

Myeloperoxidase Stain WHEN Used to differentiate AML and AMML from ALL

12

Peroxidase activity is ___ in Mature neutrophils in CML

inc

13

Peroxidase activity is ___ in Infection, AML, myelodysplastic conditions

decreased

14

Differentiate acute leukemias that are myeloid (M1, M2. M3) from monocytic origin (M5)

Esterases

15

Stains mucoproteins, glycoproteins, and HMW carbohydrates in all cells except pronormoblast


Periodic Acid-Schiff

16

Used for the diagnosis of ALL, some subtypes of AML, and DiGuglielmo’s disease

Periodic Acid-Schiff

17

PAS Reactions
Granulocytes

Intensity of stain increases as cells mature

18

PAS Reactions
Megakaryocytes

Finely diffuse stain

19

PAS Reactions
Platelets

Intensely red pink

20

PAS Reactions
M6
Early erythroblast –

course and granular

21

PAS Reactions
M6 Late erythroblast –

diffusely positive

22

Lipids like sterols, phospholipids, neutral fats in monocytes and granulocytes

Sudan Black B Stain

23

WHAT STAIN
Hairy cell leukemia



Acid phosphatase stain (with tartrate resistance

24

WHAT STAIN
Differentiate granulocytic from monocytic leukemia

Nonspecific esterase stain (with fluoride inhibition)

25

WHAT STAIN
Esterases in granulocytic cells

Chloroacetate esterase stain

26

WHAT STAIN
Screening procedure for metabolic defects in neutrophil

Nitroblue tetrazolium (NBT)

27

Dec LAP Activity

CML - Chronic Myelogenous Leukemia
Sickle Cell Anemia
Sideroblastic Leukemia
Paroxysmal Nocturnal Hemoglobinuria
Marked eosinophilia
Infectious Mononucleosis

28

Inc LAP Activity (LIP PC MOHAM)

Leukemoid reaction
Infection
pregnancy (last trimester)
polycythemia vera
corticosteroid therapy
myelosclerosis
obstructive jaundice
hodgkin's disease
aplastic leukemia
multiple myeloma

29

Normal LAP activity (LUVS)

Untreated hemolytic anemia
Lymphosarcoma
Viral hepatitis
Secondary erythrocytosis

30

LAP Score
0

No staining

31

LAP Score
1+

faint and diffuse staining

32

LAP Score
2+

pale with moderate blue staining

33

LAP Score
3+

strong blue precipitated staining

34

LAP Score
4+

deep blue or brilliant staining with no visible cytoplasm

35

Myeloperoxidase Stain stains granules in which cells

neutrophils, eosinophils and monocytes

36

appearance of basophil and lymphocyte with myeloperoxidase stain

unstained

37

Periodic Acid-Schiff Stains _____ (3) in all cells except pronormoblast

mucoproteins, glycoproteins, and HMW carbohydrates

38

PAS used in the dx of (3)

ALL, some subtypes of AML, and DiGuglielmo’s disease

39

PAS Rxns of lymphocytes in all

vary staining pattern, may be coarse or fine diffuse

40

PAS rxns of L3 or Burkitt

PAS negative

41

PAS negative

L3 or Burkitt

42

Sudan Black B stain stains Lipids like sterols, phospholipids, neutral fats in what cells (2)

monocytes and granulocytes

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