HEMA LAB 13-16 Flashcards
(61 cards)
How much NaCl do you dissolve in 100 mL of d.water (OFT)
0.5gm
color of partial hemolysis
faintly pink
color of complete hemolysis
red with no sediments
normally, hemolysis begins at what tube?
Tube 17
What kind of method did we perform in the laboratory for OFT
griffin-sanford method
other methods of OFT
- rapid screening test
- dacie’s method
- quantitative test
- unopette method
in the rrapid screening test of OFT, place 5 mL of 0.5% ____ _______ solution in a small test tube
sodium chloride
True or false. If there is hemolysis in the rapid screening, we proceed with a confirmatory test
true
In Dacie;s method, blood is diluted with what kind of solution (OFT)
isotonic saline
In Dacie;s method, blood is buffered at what pH with phosphate
7.4
In Dacie;s method, degree of hemolysis is determined on the basis of what kind of hemoglobin
free hemoglobin,
how many tubes for dacie’s method
12
time and temp for dacie’s method
30 minutes, room temp
absorbance to be used (dacie’s method)
545 nm
what is used as blank in the dacie’s method
supernatant from 0.85% saline soln
what method of OFT is used for a definitve diagnosis of hereditary spherocytosis?
quantitative test
what are carefully controlled and frequently required in the quantitative test
salt concentration and pH
method of OFT that involves the addition of blood into prepared vials containing varying concentration of NaCl
Unopette MEthod
true or false. red cells will begin to hemolyze as the %age of NaCl decreases
false…increases dapat
sino asawa ni donald duck?
dacie duck…
measures the ability of small blood vessels to control bleeding after injury
bleeding time
a measure of the function of platelets as well as the integrity of the vessel wall
duration of bleeding
what can hasten the bleeding time in duke’s method
when the filter paper touches the wound (so do not allow it)
blot the blood using filter paper every how many seconds
30
