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Flashcards in Hema Lab Deck (72):
0

determines percentage of each type of WBC

Diff White Cell Count

1

other name for staining jar method

dip method

2

staining procedures in dip method

methanol > eosin > methylene blue > buffer

3

purpose of methanol in staining method

fixative

4

good starting point for diff count

not too overlapping not too scanty

5

NV for relative seg count

50-70

6

NV for relative lymph count

18-42

7

NV for relative band count

0-5

8

NV for relative monocyte

2-11

9

NV for relative eosinophil count

1-3

10

NV for relative basophil count

0-2

11

staining which involves placing the blood smear on a rack positioned in a dish

Staining Dish method

12

6 stations in hemastainer Automatic Slide Stainer

methanol
Wright Giemsa
stain-buffer
deionized water
phosphate buffer
warm air

13

tubings in Hema-Tek 1000 slide stainer

stain
buffer
rinse

14

composition of Wright stain

oxidized methylene blue
eosin azures

15

most satisfactory in general routine hematologic studies

Wright stain

16

excellent stain for demonstrating inclusion bodies and intracellular parasites

Giemsa stain

17

components of Giemsa stains

Eosin Y with azure blue
methylene blue in methanol with glycerin

18

similar to Wright's stain

Leishman, Jenner and May-Grunwald

19

consists of romanowsky stain and another dye

panoptic stains

20

hemoglobin is measured as _____

oxyhemolobin

21

principle is conversion of hemoglobin to acid hematin with 0.1N HCl

Acid hematid method

22

rgt in cyanmetHB method

Drabkin's

23

composition of Drabkin's

sodium bicarbonate
potassium cyanide
potassium ferricyanide

24

what convert iron from ferrous to ferric state

ferricyanide

25

converts metHb to cyanmetHb

potassium cyanide

26

NV of Hb in male

14-18

27

NV of Hb in females

12-15

28

other methods of Hb det

Tallquist
Dares Hbometer
Alkaline hematin
Van Slyke oxygen capacity

29

other name for Hct

PCV
EVF

30

conversion factor for Hct

0.01

31

conversion factor for Hb

10

32

NV for Hct in males

40-54

33

NV of Hct in females

35-49

34

red capillary tube is ____

heparinized

35

common method for Hct det

Adam's method

36

other methods for Hct det

Wintrobe
Haden's
Van Allen
Sanford-Magath
Bray's

37

anticoagulant for Wintrobe Hct det

double oxalate

38

anticoag fir Haden's Hct det

1.1% sodium oxalate

39

computation for Hct% in Haden's method

colume of packed RBCs x 20

40

anticoag for Van Allen Hct method

1.6% sodium oxalate

41

anticoag for Sanford-Magath method

1.3% sodium oxalate

42

anticoag for Bray's Hct method

heparin

43

sinilarly calibrated with Wintrobe

Bray's tube

44

speed of settling of RBCs in anticoag blood

ESR

45

1st period of ESR

rouleaux formation

46

2nd period of ESR

rapid settling

47

3rd period of ESR

final period of packing

48

NV of male ESR Wintrobe

0-10mm/hr

49

NV of female ESR Wintrobe

0-20mm/hr

50

most sensitive method of ESR det for serial study of cheonic diseases

Westergren method

51

NV for Westergren ESR male

0-15

52

NV for Westergren female ESR

0-17

53

anticoag for Westergren

3.8% Trisodium citrate

54

anticoag for Wintrobe

Paul-Heller
Double oxalate

55

other ESR det mtd

Graphic or Cutler
Linzenmeier
Bray's
Micro landau
Smith micro
Crista or Hellige-Vollmer
Automated ESR syatem by Vega Biomedical

56

determination in automated ESR system by Vega takes _____ to finish

22mins

57

anticoag for Cutler ESR mtd

3% sodium citrate

58

anticoag for Linzenmeier ESR mtd

3% sodium citrate

59

anticoag for Bray's ESR mtd

1.3% sodium citrate

60

anticoag for micro landau esr mtd

5% sodium citrate

61

ESR det used for infants when veni is not practical

smith micro

62

anticoag for Vega biomedical

liquid sodium citrate

63

samples tested for Mini-Ves

4 samples

64

samples for Ves-Matic

20samples

65

samples for Ves-Matic 60

60 samples

66

network of basophilic materials

reticulum

67

index for bone marriw activity andd rbc production

retic count

68

automated method for retic count

Sysmex R-1000

69

this automated retic count uses flow cytometry

Sysmex R-1000

70

NV for adult retics

0.5-1.5%

71

NV for babies retic

2-6%