HEMA LAB - Staining Flashcards Preview

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Flashcards in HEMA LAB - Staining Deck (23):
1

other methods of staining
placing blood smear on a rack

staining dish method

2

automated method of staining 3 kinds

hemasteiner automatic slide stainer
hema-tek 1000 slide stainer
hema-tek 2000 slide stainer

3

automated method
freshly prepared staining sol'n used daily or every 4-8 hours

hemasteiner automatic slide stainer

4

automated method
bottles of stain-pak are opened by making a small hole and a cannula is inserted into each solution

hema-tek 1000 slide stainer

5

automated method
innovation: use of new pumps and volumee controls

hema-tek 2000 slide stainer

6

2 types of stain used in diff count

romanowsky stain and panoptic stain

7

type of stain used in diff count
contains methylene blue or its oxidative product, azure B
also contains eosin Y or eosin B

romanowsky stain

8

type of stain used in diff count
dyes produce multiple colors when used on cells

romanowsky stain

9

type of stain used in diff count
polychromatic stain

romanowsky stain

10

types of romanowsky stain [3]

Wright's
Giemsa
Leishman, Jenner, May-Grunwald

11

types of romanowsky stain
most satisfactory

wright's stain

12

types of romanowsky stain
for demo on wbc, inclusion bodies, intracellular parasites

giemsa

13

types of romanowsky stain
similar to wright's stain except for the method used to oxidize methylene blue

Leishman, Jenner, May-Grunwald

14

consist of romanowsky stain and another dye

panoptic stain

15

panoptic stains [3]

Wright's-Giemsa
Jenner-Giemsa
May-Grunwald-Giemsa

16

methods of smear prep [3]

two-slide or wedge method
two coverslip or ehrlich's two-coverglass
spinner's method

17

spinner's method is done using an instrument called

hemaspinner

18

in spinner's method, the clean glass is placed on a --

platen

19

modern spinners contain __ to detect if cells have separated properly. this prompts the platen to automatically stop spinning

optical system with sensor

20

instrument for automated slide-making and staining of blood
smears prep after analysis of blood sample

sysmex SP-1000i

21

in sysmex SP-1000i smears are prepared every --

30 sec

22

amount of blood, angle, speed of spreader in sysmex SP-1000i dependent on --

Hct value

23

another instrument aside from sysmex SP-1000i for smear making and analyzing

beckman coulter blood analyzer

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