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Flashcards in Hemoglobin Measurement Deck (11)
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0

Other term for Drabkin's reagent

Potassium ferricyanide-cyanide solution

1

Precautions in hemoglobin measurement

1. Salts and solution of cyanide are poisonous. Care should be taken while handling such salts and solution.
2. Drabkin's solution is sensitive to light and should be stored in a brown bottle and in the dark.
3. Drabkin's solution contains cyanide. When filling pipettes, a suction bulb should be used.
4. Drabkin's solution should not be used after precipitate has formed in the bottom of the storage bottle.

2

It is measured photometrically.

Intensity of cyanmethemoglobin

3

Other term for hemoglobin measurement

Cyanmethemoglobin method

4

Formula for hemoglobin concentration

Hb (g/dL) = 36.8 x Abs

5

Action of Drabkin's solution

Oxidizes hemoglobin to methemoglobin which is in turn converted to cyanmethemoglobin

6

Set wavelength at ______

540 nm

7

Allow the tubes to stand for _______

10 minutes

8

Components of the reagent

1 g of sodium bicarbonate
0.52 g of potassium cyanide
0.198 g of potassium ferricyanide

9

Sample used for hemoglobin measurements

Whole blood

11

Hemoglobin normal range

Female 12-16 g/dL
Male 14-18 g/dL