Flashcards in Hemoglobin Measurement Deck (11)
Other term for Drabkin's reagent
Potassium ferricyanide-cyanide solution
Precautions in hemoglobin measurement
1. Salts and solution of cyanide are poisonous. Care should be taken while handling such salts and solution.
2. Drabkin's solution is sensitive to light and should be stored in a brown bottle and in the dark.
3. Drabkin's solution contains cyanide. When filling pipettes, a suction bulb should be used.
4. Drabkin's solution should not be used after precipitate has formed in the bottom of the storage bottle.
It is measured photometrically.
Intensity of cyanmethemoglobin
Other term for hemoglobin measurement
Formula for hemoglobin concentration
Hb (g/dL) = 36.8 x Abs
Action of Drabkin's solution
Oxidizes hemoglobin to methemoglobin which is in turn converted to cyanmethemoglobin
Set wavelength at ______
Allow the tubes to stand for _______
Components of the reagent
1 g of sodium bicarbonate
0.52 g of potassium cyanide
0.198 g of potassium ferricyanide
Sample used for hemoglobin measurements