Homework 4 questions Flashcards
The lac operon encodes a polycistronic mRNA. What DNA or protein components is there multiple of in the operon
ribosomal binding sites
in conditions with high glucose AND high lactose, the lac operon will be … because …
mostly off because the presence of glucose leads to inhibition of LacY
what is not involved in regulating the trp operon
regulatory sRNA and transcriptional interference
what is involved with regulating the trp operon
abundance of charged tRNA (trp), a repressor protein that blocks transcription, transcriptional attenuation
what is the location of the attenuator region that controls the expression of the trp operon
it is between the transcription start site and the first structural gene
bacteria would most likely use … to make small gene expression changes in response to a single environmental stressor, and … to make large readjustments to gene expression in response to multiple simultaneous stressors
a transcriptional regulator and an alternative sigma factor
an RNA thermometer is used to control … in response to …
translation and temperature
what is not true about second messenger molecules
they are advantageous because they can act as biosynthetic precursors and regulators
what is true about second messengers
many are derivatives of nucleotides, they can regulate by binding to RNA and proteins, they can signal within cells to regulate multiple operons
for a cis-asRNA regulating by transcription interference, you would expect it to function normally if the asRNA was instead encoded in trans
False
a <3,000 nt RNA molecule that is transcribed from the non-template DNA strand of a protein-coding gene and regulates only that gene is classified as an…
asRNA
abundant diguanylate cyclases and very little phosphodiesterase enzymes are produced. what are the c-di-GMP concentrations and what does that promote
high and biofilm formation
what is EnvZ … that modifies OmpR which is a …
a dual function kinase and phosphatase and a response regulator
RpoS is produced … and kept at very low levels at other times due to …
in stationary phase and RNase III cleavage of mRNA and protein degradation
what describes the key difference between QS systems in gram-positive and gram-negative
the chemistry of the autoinducer requires different uptake mechanims
