Immunochemical Techniques Flashcards Preview

Chemistry Module 4 > Immunochemical Techniques > Flashcards

Flashcards in Immunochemical Techniques Deck (33):
1

Competitive formats use ________ reagents (antibody or other antigen binder)

LIMITED

2

Noncompetitive formats use _______ reagents (antibody or other antigen binder) and are two-site or "________" assays

EXCESS
sandwich

3

What does it mean that competitive immunoassays are simultaneous?

all reactants are mixed together simultaneously or at the same time

4

Simultaneous Competitive Immunoassay:
- Principle

Labled and unlabeled antigen compete for a limited number of binding sites on an antibody binder. Avidity for the antibody is the same for both.

5

Simultaneous Competitive Immunoassay:
- Proportionality observed between bound able and unlabeled antigen concentrations

The probability of labeled Ag binding to the antibody binder is inversely proportional to (unlabeled Ag)

6

Simultaneous Competitive Immunoassays
- Examples of tests

RIA, FIA, EIA

7

Noncompetitive Immunoassay:
- Principle

A captured antibody is bound to a solid phase. Unlabeled antigen reacts with the solid phase antibody, then there's a washing step. Labeled antibody is added; it reacts with the unbound antigen on a 2nd and distinct epitope. Another washing step occurs, and the amount of the bound label is determined

8

Noncompetitive Immunoassay:
- Proportionality observed between bound able and unlabeled antigen concentrations

directly proportional

9

What is the difference of a heterogeneous immunoassays from homogeneous immunoassays?

Heterogeneous require a separation step to separate the bound label from the free, unbound reactants. Homogeneous do not require a separation step

10

EMIT:
- what does it stand for

Enzyme-Multiplied Immunoassay Technique

11

EMIT:
- What is detected

change in absorbance after adding substrate

12

EMIT:
- Homogeneous or heterogeneous

Homogenous

13

EMIT:
- Proportionality

direct

14

ELISA:
- what does it stand for

Enzyme-Linked Immunosorbent Assay

15

ELISA:
- what is detected

Change in absorbance

16

ELISA:
- Homogeneous or heterogeneous

Heterogeneous

17

ELISA:
- Proportionality

Direct or indirect

18

MEIA:
- What does it stand for

Microparticle Enzyme Immunoassay

19

MEIA:
- What is detected

fluorescences

20

MEIA:
- Homogeneous or Heterogenous

Heterogenous?

21

MEIA:
- Proportionality

Direct

22

FIA:
- What does it stand for

Fluorescent Immunoassays

23

FIA:
- What is detected

Fluorescence

24

FIA:
- Homogeneous or Heterogeneous

Heterogeneous

25

FIA:
- Proportionality

Direct Or Indirect

26

FPIA
- What does it stand for

Fluorescence Polarization Immunoassay

27

FPIA
- What is detected

Plane Polarized Light

28

FPIA
- Homogeneous or Heterogeneous

Homogeneous

29

FPIA
- Proportionality

indirect

30

FPIA
- most common fluorescent label

FITC

31

Chemiluminescence
- what is detected

Visible light produced from labels after a base is added are measured by luminometer

32

Chemiluminescence
- Proportionality

direct

33

Chemiluminescence
- two common labels

Luminol or acridinium ester