Immunological and Molecular Diagnostics

Question 1

Markers of innate immunity?

Answer 1

Acute phase proteins

Question 2

Markers of adaptive immunity?

Answer 2

Cytokines and antibodies

Question 3

What does sandwich ELISA detect and when would you use it?

Answer 3

Antigen - <5D post exposure

Question 4

What does indirect ELISA detect?

Answer 4

Antibodies >5 post exposure

Question 5

How is a virus neutralisation assay performed?

Answer 5

Cultured cells deliberately infected and observed for cytopathic effects

Question 6

Give an example of a disease that can be detected using a delayed-type hypersensitivity test..

Answer 6

bTB

Question 7

What method is most commonly used to detect pathogen DNA/RNA in a sample?

Answer 7

PCR/ qPCR

Question 8

What is the specific test for IMHA?

Answer 8

Coomb’s test

Question 9

What is the specific test for SLE?

Answer 9

ANA (anti-nuclear antibody)

Question 10

What is the test for acquired myaesthenia gravis?

Answer 10

AChR autoantibodies

Question 11

Examples of acute phase proteins?

Answer 11

C-reactive protein
Serum amyloid A
Fibrinogen
Haptoglobin