Introduction to Micro anatomy Flashcards Preview

FMS Week 5 > Introduction to Micro anatomy > Flashcards

Flashcards in Introduction to Micro anatomy Deck (12):
1

Tissue Preparation: Fixation

Purpose: to preserve tissues and prevent tissue degredtion

Small pieces placed into chemial solutions

Large organs or whole bodies have blood vessels infused with chemicals

2

Fixative for light microscopy 

Formalin

3

Fixative for electron microscopy

glutaraldehyde 

4

Tissue Preparation: Embedding and Sectioning

Purpose: to allow tissue to be sliced thinly enough to be examined by microscopy 

5

Embedding for light microscopy

Paraffin

6

Embedding for electron microscopy

plastic resins 

7

Tissue preparation: Staining

Purpose: allows cells and ECM to be seen

8

H&E stain

Routine Exam

Hematoxylin is a dark blue voilet stain. Is basophilic/ positive. Binds to acidic/ - such as DNA/RNA

Eosin is red/pink counterstain. Is acidic/ -. Binds to acidophiolic + substances such as cytoplasmic proteins

9

Gram Stain

To determine if/ what kind of bacteria present in a substance.

Tissue stained with crystal viollet dye and Grams iodine solution, Then decolorized and counterstained.

Gram + violet bc thick peptidoglycan wall

Gram - take up pink counteratain

10

Wright Geimsa Stain

To study blood cells. 

Combo of stains including methylene blue, azure B, and eosin

Eosin stains cytoplasm pink

Methylene blue and Azure B stain nucleus and components varying shades of blue and purple

11

Immunohistochemistry

Uses labeled antibodies. 

Most often to investigate infection and neoplasia 

Brown stain

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