Tissue Preparation: Fixation
Purpose: to preserve tissues and prevent tissue degredtion
Small pieces placed into chemial solutions
Large organs or whole bodies have blood vessels infused with chemicals
Fixative for light microscopy
Fixative for electron microscopy
Tissue Preparation: Embedding and Sectioning
Purpose: to allow tissue to be sliced thinly enough to be examined by microscopy
Embedding for light microscopy
Embedding for electron microscopy
Tissue preparation: Staining
Purpose: allows cells and ECM to be seen
Hematoxylin is a dark blue voilet stain. Is basophilic/ positive. Binds to acidic/ - such as DNA/RNA
Eosin is red/pink counterstain. Is acidic/ -. Binds to acidophiolic + substances such as cytoplasmic proteins
To determine if/ what kind of bacteria present in a substance.
Tissue stained with crystal viollet dye and Grams iodine solution, Then decolorized and counterstained.
Gram + violet bc thick peptidoglycan wall
Gram - take up pink counteratain
Wright Geimsa Stain
To study blood cells.
Combo of stains including methylene blue, azure B, and eosin
Eosin stains cytoplasm pink
Methylene blue and Azure B stain nucleus and components varying shades of blue and purple
Uses labeled antibodies.
Most often to investigate infection and neoplasia