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Flashcards in Introduction to microbiology Deck (25)
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1
Q
what is the 5 possible infecting agents
A
Bacteria
Viruses
Fungi
Parasites
Prions
2
Q
What is a prion
A
type of protein that can trigger normal proteins in the brain to fold abnormally
3
Q
What specimen type is collected for culture in Meningitis
A
cerebro spinal fluid
4
Q
What specimen type is collected for culture in Bacteraemia
A
Blood culture
5
Q
What is a bacteria in the blood called?
A
Bacteraemia
6
Q
What specimen type is collected for culture in chest infection
A
Sputum
7
Q
What specimen type is collected for culture in urinary tract infection
A
Midstream urine
8
Q
What is a gram stain used to see
A
Bacterium and yeast/fungi
9
Q
What isn't visible in light microscope
A
Viruses
10
Q
What is the difference between sterile and non sterile sites
A
Sterile easier to identify as only one organism present, where in non sterile other bacteria are present therefore harder to distinguish particular organism
11
Q
Examples of sterile sites
A
Blood
CSF
Lung
Bladder
12
Q
Examples of non-sterile sites
A
Skin
Nasopharynx
Urethra
Gut
13
Q
Methods used for Diagnosis of viral infection
A
PCR
Antigen detection
Cell/tissue culture inoculation
serology
Electron Microscopy
14
Q
Diagnosis viral infection for malaria parasite
A
Microscopy of blood films
15
Q
Diagnosis viral infection in your intestinal tract
A
parasites, cysts and ova in faeces
16
Q
What is PCR
A
Multiple copies of DNA are made by the repeated action of polymerase
17
Q
What is serology
A
Testing blood serum for immunity in repose to pathogen
18
Q
Characteristic of Gram stain
A
Rapid and not sensitive
19
Q
What does unstained microscopy allow you to see
A
Pus cells (urine CSF)
Parasites (faeces)
20
Q
What does ZN stain or auramine stain allow you to see
A
myobacteria (a bacterium of a group which includes the causative agents of leprosy and tuberculosis)
21
Q
What is the benefits of culture medium compared to microscopy
A
Conditions are suitable for expected species
and can identify particular species
22
Q
What is the benefit of microscopy to culture medium
A
Microscopy is rapid and insensitive
23
Q
How is a species identified
A
By its characteristics
DNA tests
Typing
24
Q
What does typing do
A
determines strain within a species, by creating DNA profile
25
Q
Examples of health care acquired infections
A
MRSA - cause blood poisoning
Clostridium difficile - infect the bowel and cause diarrhoea
Norovirus - stomach bugs

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