Investigate photosynthesis using isolated chloroplasts, Hill reaction (11)

Question 1

Controlled variables:

Answer 1

Temperature
Volume of chloroplast extract
Time exposed to light and light intensity

Question 2

Reasons why isolation medium that’s mixed with leaf extract is cold, has buffer and sucrose solution:

Answer 2

Cold: Slows down enzyme activity
Buffer: Ensures pH is at 7 (neutral) so enzymes (RUBISCO) don’t denature
Sucrose: Stops osmotic loss of water from chloroplasts