Kandpal-Translation Initiation Flashcards Preview

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Flashcards in Kandpal-Translation Initiation Deck (32):
1

where do ribosomes bind?

at a conserved 5' untranslated region (UTR) 10 bases upstream of AUG

2

what is the name of where the ribosome binds?

shine-dalgarno sequence (AGGAGGU)

3

what does the initiator tRNA contain?

N-formyl-methionine (fMet)

4

what type of immune response remembers previous encounter with pathogens?

adaptive immune system

5

what type of immune response relies on proteins and phagocytic cells to recognize pathogens?

innate immunity

6

what type of peptide does the body use to distinguish self from non-self?

formylmethionine containing peptides

7

what initiation factors keep the 30S subunit free?

IF-1 and IF-3

8

what initiation factor blocks the A site?

IF-1

9

what initiation factor is important for binding mRNA?

IF-3

10

what factor allows for tRNA to bind to 30S subunit?

IF-2

11

when will the 50S and 30S subunit combine?

when IFs are released

12

what enters the partial P site on 30S subunit bound to mRNA?

fMet-tRNA

13

what part of the EF-Tu (elongation factor) complex enters the A site?

alpha,alpha-tRNA

14

where the is fmet from the initiator-tRNA transferred too?

to the aminoacyl-tRNA in the A site

15

what part of the ribozyme contains the peptidyl transferase activity?

23 rRNA of the large subunit

16

what causes the exit of the tRNA from P site?

GTP binding elongation factor EF-G

17

do tRNA's recognize stop codons?

nope

18

what events do release factors trigger?

cleavage of polypeptide from tRNA, release of polypeptide and mRNA and dissociation of ribosome subunits

19

how do antibiotics inhibit protein synthesis?

they bind selectively to various sites on ribosomes

20

what does choloramphenicol do in bacteria?

inhibits peptidyl transferase in prokaryotes (50S)

21

what does streptomycin do?

inhibits initiation to elongation transition (30S)

22

what does erythromycin do in bacteria?

inhibits translocation by binding to 50S ribosome

23

what does tetracycline do in bacteria?

prevents aminoacyl-tRNA binding to A site (30S)

24

in eukaryotic translation, what is unique about the tRNA that is used to initiate synthesis?

contains its own aminoacyl tRNA synthetase

25

in eukaryotes does f-Met still serve a function in the tRNA?

no. only Met can interact with eIF2 not f-Met

26

what is needed to help the intiation complex unwind and scan the mRNA?

ATP

27

what does eIF4 bind too?

"cap", Poly(A), mRNA, 40S ribosomal subunit

28

does eIF4 have helicase activity?

yes to unwind the secondary structure

29

when will translation take place in the eukaryotes?

when the intiation complex moves from the 5'-end cap to the first AUG

30

what initiation complex is formed in eukaryotes when 40S initiation complex joins the 60S subunit?

80S ribosome

31

what allows for elongation in eukaryotes?

GTP binding EF1-alpha

32

hydrolysis of what is necessary for translocation to take place (move from A site to P site)?

EF2-GTP