Flashcards in lab 1 Deck (15):
How do you dispose of tubes?
Upright in basket, caps half turned, and remove all tape.
How do you dispose of petri dishes?
Leave labels and tape on hinges, they go upright on bottom of plastic cart.
What magnification is the objective lens and what is the total magnification?
What magnification is the low power objective lens and what is the total magnification?
What magnification high-dry objective lens and total magnification?
What magnification is the oil immersion and total magnification?
How do you find total magnification?
Objective x ocular (10x) = total magnification
What are the 5 steps for focusing a specimen?
1. start with 4x
2. raise stage all the way up using coarse adjustment knob
3. lower stage with fine adjustment knob until object is focused
4. center object in middle field of view and change to 10x
5. refocus as needed
When and why is oil immersion used?
-Always used when viewing bacteria
-Used only when 100x objective is required for observation.
What is a TEM microscope?
-Used to observe fine details of cell structure (inside cell)
-Generates a beam of electrons that produce an image on a fluorescent screen
What is a SEM microscope?
-Uses a magnetic field within a vacuum tube to manipulate a beam of electrons
-surface of cells can be observed
What can be seen with a scanning probe microscope?
-Small molecules, typically DNA
What is resolution?
The ability to clearly separate 2 objects that are closely together.
How does immersion oil improve resolution?
Without oil there is air space that scatters the light. The oil traps the light which keeps the resolution clear.