Flashcards in Lab: Bright field, Phase contrast, and Fluorescence Microscopy Deck (10):
3 typical bacterial morphologies
1. chains (streptococci)
2. clusters (staphylococci)
3. diplococcus (pairs of balls)
What is a bright field microscope?
allows light rays to pass directly to the eye without deflection from an opaque plate in the condenser
What does a condenser do?
collects and directs light from the lamp to the slide
Bright field microscopes are parfocal which means
you can change from a lower objective to a higher objective while keeping the image in focus
In Bright field microscopy, the sample must be ________ in order to see it
How is total magnification found?
objective * ocular
What are the 4 types of objectives?
4x/5x rapid scanning
10x low power
40x/50x high power
100x oil immersion
What is a phase contrast microscope?
Able to differentiate transparent structures of a microorganism and enhance the contrast between the cell and its surroundings through the use of an opaque disc in the condenser
With a phase contrast microscope the specimen can be ________