Lab: Bright field, Phase contrast, and Fluorescence Microscopy Flashcards Preview

Microbiology > Lab: Bright field, Phase contrast, and Fluorescence Microscopy > Flashcards

Flashcards in Lab: Bright field, Phase contrast, and Fluorescence Microscopy Deck (10):
1

3 typical bacterial morphologies

1. chains (streptococci)
2. clusters (staphylococci)
3. diplococcus (pairs of balls)

2

What is a bright field microscope?

allows light rays to pass directly to the eye without deflection from an opaque plate in the condenser

3

What does a condenser do?

collects and directs light from the lamp to the slide

4

Bright field microscopes are parfocal which means

you can change from a lower objective to a higher objective while keeping the image in focus

5

In Bright field microscopy, the sample must be ________ in order to see it

stained

6

How is total magnification found?

objective * ocular

7

What are the 4 types of objectives?

4x/5x rapid scanning
10x low power
40x/50x high power
100x oil immersion

8

What is a phase contrast microscope?

Able to differentiate transparent structures of a microorganism and enhance the contrast between the cell and its surroundings through the use of an opaque disc in the condenser

9

With a phase contrast microscope the specimen can be ________

living

10

What is a fluorescent microscope?

Used primarily in the immune-medical field and microbial ecology. One of the most common approaches used in studying the location and movement of molecules and cellular components within the cell.